PUBLICATION

The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen

Authors
Sander, V., Patke, S., Lee, J.Y., Chang, Y.T., Davidson, A.J.
ID
ZDB-PUB-170326-18
Date
2017
Source
Molecules   22(3): (Journal)
Registered Authors
Davidson, Alan
Keywords
CDr10b, gut development, laser ablation, mid-intestine, vital dye, zebrafish
MeSH Terms
  • Animals
  • Boron Compounds/administration & dosage*
  • Intestines/ultrastructure*
  • Larva/anatomy & histology
  • Microscopy, Fluorescence
  • Signal Transduction
  • Tretinoin/metabolism
  • Zebrafish/anatomy & histology*
  • Zebrafish/growth & development
PubMed
28335401 Full text @ Molecules
Abstract
We describe the use of the fluorescent reporter compound CDr10b to label mid-intestinal structures in zebrafish larvae after simple immersion. CDr10b is deposited into the gut where it initially fills the lumen and is excreted. Using laser-mediated injury of the intestine, we show that CDr10b provides a useful readout of the integrity and repair of the epithelial cell barrier. In addition, CDr10b specifically labels the absorptive mid-intestine segment that is analogous to the mammalian small intestine. By perturbing retinoic acid signaling, which regulates the size of the mid-intestine segment, we show that CDr10b is a valuable tool to rapidly assess developmental malformations of the intestine in live animals.
Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes