The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen
- Sander, V., Patke, S., Lee, J.Y., Chang, Y.T., Davidson, A.J.
- Molecules 22(3): (Journal)
- Registered Authors
- Davidson, Alan
- CDr10b, gut development, laser ablation, mid-intestine, vital dye, zebrafish
- MeSH Terms
- Boron Compounds/administration & dosage*
- Larva/anatomy & histology
- Microscopy, Fluorescence
- Signal Transduction
- Zebrafish/anatomy & histology*
- Zebrafish/growth & development
- 28335401 Full text @ Molecules
Sander, V., Patke, S., Lee, J.Y., Chang, Y.T., Davidson, A.J. (2017) The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen. Molecules. 22(3).
We describe the use of the fluorescent reporter compound CDr10b to label mid-intestinal structures in zebrafish larvae after simple immersion. CDr10b is deposited into the gut where it initially fills the lumen and is excreted. Using laser-mediated injury of the intestine, we show that CDr10b provides a useful readout of the integrity and repair of the epithelial cell barrier. In addition, CDr10b specifically labels the absorptive mid-intestine segment that is analogous to the mammalian small intestine. By perturbing retinoic acid signaling, which regulates the size of the mid-intestine segment, we show that CDr10b is a valuable tool to rapidly assess developmental malformations of the intestine in live animals.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes