PUBLICATION
Video-rate volumetric functional imaging of the brain at synaptic resolution
- Authors
- Lu, R., Sun, W., Liang, Y., Kerlin, A., Bierfeld, J., Seelig, J.D., Wilson, D.E., Scholl, B., Mohar, B., Tanimoto, M., Koyama, M., Fitzpatrick, D., Orger, M.B., Ji, N.
- ID
- ZDB-PUB-170303-11
- Date
- 2017
- Source
- Nature Neuroscience 20: 620–628 (Journal)
- Registered Authors
- Orger, Mike
- Keywords
- Biological fluorescence, Fluorescence imaging, Optical imaging
- MeSH Terms
-
- Animals
- Axons
- Brain/physiology*
- Calcium/metabolism
- Dendrites/physiology
- Drosophila melanogaster
- Imaging, Three-Dimensional/methods*
- Mice
- Microscopy, Confocal
- Neural Inhibition/physiology
- Neurons/physiology
- Photons
- Synapses/physiology*
- Zebrafish
- PubMed
- 28250408 Full text @ Nat. Neurosci.
Citation
Lu, R., Sun, W., Liang, Y., Kerlin, A., Bierfeld, J., Seelig, J.D., Wilson, D.E., Scholl, B., Mohar, B., Tanimoto, M., Koyama, M., Fitzpatrick, D., Orger, M.B., Ji, N. (2017) Video-rate volumetric functional imaging of the brain at synaptic resolution. Nature Neuroscience. 20:620–628.
Abstract
Neurons and neural networks often extend hundreds of micrometers in three dimensions. Capturing the calcium transients associated with their activity requires volume imaging methods with subsecond temporal resolution. Such speed is a challenge for conventional two-photon laser-scanning microscopy, because it depends on serial focal scanning in 3D and indicators with limited brightness. Here we present an optical module that is easily integrated into standard two-photon laser-scanning microscopes to generate an axially elongated Bessel focus, which when scanned in 2D turns frame rate into volume rate. We demonstrated the power of this approach in enabling discoveries for neurobiology by imaging the calcium dynamics of volumes of neurons and synapses in fruit flies, zebrafish larvae, mice and ferrets in vivo. Calcium signals in objects as small as dendritic spines could be resolved at video rates, provided that the samples were sparsely labeled to limit overlap in their axially projected images.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping