PUBLICATION

Effect of environmentally-relevant concentrations of nonylphenol on sexual differentiation in zebrafish: a multi-generational study

Authors
Sun, D., Chen, Q., He, N., Diao, P.P., Jia, L.X., Duan, S.S.
ID
ZDB-PUB-170224-6
Date
2017
Source
Scientific Reports   7: 42907 (Journal)
Registered Authors
Keywords
Ecology, Ecophysiology
MeSH Terms
  • Animals
  • Aromatase/genetics
  • Aromatase/metabolism
  • Body Size/drug effects
  • Endocrine Disruptors/toxicity*
  • Environmental Exposure
  • Estrogen Receptor alpha/genetics
  • Estrogen Receptor alpha/metabolism
  • Female
  • Male
  • Phenols/toxicity*
  • Sex Differentiation/drug effects*
  • Sex Differentiation/genetics
  • Sex Ratio
  • Up-Regulation/drug effects
  • Zebrafish/growth & development
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
28230098 Full text @ Sci. Rep.
Abstract
Nonylphenol (NP) is a persistent environmental chemical that can disrupt the organism's endocrine system, and is detected in the surface water and sea. In this study, we investigated whether NP can alter transcriptional expression of sexual differentiation-related genes. Three generations of zebrafish were exposed to 0, 2, 20 and 200 μg·L-1 of NP, and transcriptional expression of sexual differentiation genes were assessed in 10, 20 and 40 dpf in the F1 and F2 generations. Growth of zebrafish exposed to 200 μg·L-1 of NP was inhibited at 125 dpf in the F1 generation. 20 μg·L-1 of NP resulted in 80% females in the F1 generation, but had no effect on the F2 generation. In terms of the sexual differentiation genes, the transcriptional expression of cyp19a1a and esr1 genes were upregulated in 20 μg·L-1 of NP in the F1 generation. But expression of the sexual differentiation genes were not affected in the F2 generation. Overall, NP could affect sexual differentiation and gene transcriptional expression in the F1 generation. The tolerance of contaminant in the offsprings was improved at low concentration.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping