PUBLICATION
A Mixture of Persistent Organic Pollutants and Perfluorooctanesulfonic Acid Induces Similar Behavioural Responses, but Different Gene Expression Profiles in Zebrafish Larvae
- Authors
- Khezri, A., Fraser, T.W., Nourizadeh-Lillabadi, R., Kamstra, J.H., Berg, V., Zimmer, K.E., Ropstad, E.
- ID
- ZDB-PUB-170202-2
- Date
- 2017
- Source
- International Journal of Molecular Sciences 18(2): (Journal)
- Registered Authors
- Nourizadeh-Lillabadi, Rasoul
- Keywords
- PFOS, behavioural, neurotoxicity, persistent organic pollutants, zebrafish larvae
- MeSH Terms
-
- Alkanesulfonic Acids/adverse effects*
- Animals
- Behavior, Animal/drug effects*
- Cluster Analysis
- Fertilization
- Fluorocarbons/adverse effects*
- Gene Expression Profiling
- Gene Expression Regulation/drug effects*
- Larva/drug effects
- Organic Chemicals/adverse effects*
- Swimming
- Transcriptome
- Water Pollutants, Chemical/adverse effects*
- Zebrafish*/genetics
- PubMed
- 28146072 Full text @ Int. J. Mol. Sci.
Citation
Khezri, A., Fraser, T.W., Nourizadeh-Lillabadi, R., Kamstra, J.H., Berg, V., Zimmer, K.E., Ropstad, E. (2017) A Mixture of Persistent Organic Pollutants and Perfluorooctanesulfonic Acid Induces Similar Behavioural Responses, but Different Gene Expression Profiles in Zebrafish Larvae. International Journal of Molecular Sciences. 18(2).
Abstract
Persistent organic pollutants (POPs) are widespread in the environment and some may be neurotoxic. As we are exposed to complex mixtures of POPs, we aimed to investigate how a POP mixture based on Scandinavian human blood data affects behaviour and neurodevelopment during early life in zebrafish. Embryos/larvae were exposed to a series of sub-lethal doses and behaviour was examined at 96 h post fertilization (hpf). In order to determine the sensitivity window to the POP mixture, exposure models of 6 to 48 and 48 to 96 hpf were used. The expression of genes related to neurological development was also assessed. Results indicate that the POP mixture increases the swimming speed of larval zebrafish following exposure between 48 to 96 hpf. This behavioural effect was associated with the perfluorinated compounds, and more specifically with perfluorooctanesulfonic acid (PFOS). The expression of genes related to the stress response, GABAergic, dopaminergic, histaminergic, serotoninergic, cholinergic systems and neuronal maintenance, were altered. However, there was little overlap in those genes that were significantly altered by the POP mixture and PFOS. Our findings show that the POP mixture and PFOS can have a similar effect on behaviour, yet alter the expression of genes relevant to neurological development differently.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping