|ZFIN ID: ZDB-PUB-161228-3|
A Novel Tool to Measure Extracellular Glutamate in the Zebrafish Nervous System In Vivo
MacDonald, R.B., Kashikar, N.D., Lagnado, L., Harris, W.A.
|Source:||Zebrafish 14(3): 284-286 (Journal)|
|Registered Authors:||Harris, William A., Lagnado, Leon, MacDonald, Ryan|
|Keywords:||glia, glutamate, nervous system, retina|
|PubMed:||28027028 Full text @ Zebrafish|
MacDonald, R.B., Kashikar, N.D., Lagnado, L., Harris, W.A. (2017) A Novel Tool to Measure Extracellular Glutamate in the Zebrafish Nervous System In Vivo. Zebrafish. 14(3):284-286.
ABSTRACTGlutamate is the major excitatory neurotransmitter in the brain. Its release and eventual recycling are key to rapid sustained neural activity. We have paired the gfap promoter region with the glutamate reporter molecule, iGluSnFR, to drive expression in glial cells throughout the nervous system. Tg(gfap:iGluSnFR) is expressed on the glial membrane of Müller glia cells in the retina, which rapidly respond to stimulation and the release of extracellular glutamate. As glial cells are associated with most, if not all, synapses, Tg(gfap:iGluSnFR) is a novel and exciting tool to measure neuronal activity and extracellular glutamate dynamics in many regions of the nervous system.