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ZFIN ID: ZDB-PUB-161228-3
A Novel Tool to Measure Extracellular Glutamate in the Zebrafish Nervous System In Vivo
MacDonald, R.B., Kashikar, N.D., Lagnado, L., Harris, W.A.
Date: 2017
Source: Zebrafish 14(3): 284-286 (Journal)
Registered Authors: Harris, William A., Lagnado, Leon, MacDonald, Ryan
Keywords: glia, glutamate, nervous system, retina
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/growth & development
  • Animals, Genetically Modified/metabolism
  • Escherichia coli Proteins/metabolism*
  • Gene Expression Regulation
  • Glutamic Acid/metabolism*
  • Green Fluorescent Proteins/metabolism*
  • Nervous System/cytology
  • Nervous System/metabolism*
  • Neuroglia/cytology
  • Neuroglia/metabolism
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins/metabolism*
  • Retina/cytology
  • Retina/metabolism
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
PubMed: 28027028 Full text @ Zebrafish
FIGURES
ABSTRACT
Glutamate is the major excitatory neurotransmitter in the brain. Its release and eventual recycling are key to rapid sustained neural activity. We have paired the gfap promoter region with the glutamate reporter molecule, iGluSnFR, to drive expression in glial cells throughout the nervous system. Tg(gfap:iGluSnFR) is expressed on the glial membrane of Müller glia cells in the retina, which rapidly respond to stimulation and the release of extracellular glutamate. As glial cells are associated with most, if not all, synapses, Tg(gfap:iGluSnFR) is a novel and exciting tool to measure neuronal activity and extracellular glutamate dynamics in many regions of the nervous system.
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