PUBLICATION
C1qr and C1qrl redundantly regulate angiogenesis in zebrafish through controlling endothelial Cdh5
- Authors
- Du, J., Yang, Q., Luo, L., Yang, D.
- ID
- ZDB-PUB-161224-11
- Date
- 2017
- Source
- Biochemical and Biophysical Research Communications 483(1): 482-487 (Journal)
- Registered Authors
- Luo, Lingfei
- Keywords
- Angiogenesis, Zebrafish, c1qr, c1qrl, cdh5
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- CRISPR-Cas Systems
- Cadherins/genetics
- Cadherins/metabolism*
- Endothelial Cells/metabolism
- Gene Expression Regulation, Developmental
- Gene Knockout Techniques
- Larva
- Mutation
- Neovascularization, Physiologic/genetics*
- Zebrafish/genetics*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 28007601 Full text @ Biochem. Biophys. Res. Commun.
Citation
Du, J., Yang, Q., Luo, L., Yang, D. (2017) C1qr and C1qrl redundantly regulate angiogenesis in zebrafish through controlling endothelial Cdh5. Biochemical and Biophysical Research Communications. 483(1):482-487.
Abstract
Angiogenesis plays central role in the formation of functional circulation system. Characterizations of the involved factors and signaling pathways remain to be the key interest in the angiogenesis research. In this report, we showed that c1qr/cd93 and c1qrl/clec14a are specifically expressed in the vascular endothelial cells during zebrafish development. Single mutation of c1qr or c1qrl is associated with slightly malformation of inter-segmental vessels (ISVs), whereas double mutant exhibits severe defects in the ISVs formation without affecting early vasculogenesis. Further studies reveal that the endothelial-endothelial junctional molecule Cdh5 becomes absent in the ISVs of the double mutant. Replenishment of Cdh5 efficiently rescue the impaired angiogenesis in the c1qr/c1qrl double mutant. These data demonstrate that c1qr and c1qrl redundantly regulate angiogenesis through controlling the expression of the endothelial junctional molecule Cdh5, thus playing an important role in angiogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping