PUBLICATION
Effect of tributyltin on antioxidant ability and immune responses of zebrafish (Danio rerio)
- Authors
- Zhang, C.N., Zhang, J.L., Ren, H.T., Zhou, B.H., Wu, Q.J., Sun, P.
- ID
- ZDB-PUB-161218-2
- Date
- 2017
- Source
- Ecotoxicology and environmental safety 138: 1-8 (Journal)
- Registered Authors
- Keywords
- Antioxidant capability, Danio rerio, Immune response, Tributyltin
- MeSH Terms
-
- Animals
- Antioxidants/metabolism
- Catalase/metabolism
- Cytokines/metabolism
- Disinfectants/toxicity*
- Glutathione Peroxidase/metabolism
- Heat-Shock Proteins/metabolism
- Immunity/drug effects*
- Liver/drug effects
- Liver/metabolism
- Malondialdehyde/metabolism
- Muramidase/metabolism
- Oxidative Stress/drug effects*
- Random Allocation
- Superoxide Dismutase/metabolism
- Trialkyltin Compounds/metabolism
- Trialkyltin Compounds/toxicity*
- Tumor Necrosis Factor-alpha/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish*/immunology
- Zebrafish*/metabolism
- PubMed
- 27987418 Full text @ Ecotoxicol. Environ. Saf.
Citation
Zhang, C.N., Zhang, J.L., Ren, H.T., Zhou, B.H., Wu, Q.J., Sun, P. (2017) Effect of tributyltin on antioxidant ability and immune responses of zebrafish (Danio rerio). Ecotoxicology and environmental safety. 138:1-8.
Abstract
Tributyltin (TBT) is a toxic compound released into aquatic ecosystems through antifouling paints. This study was designed to examine the effects of TBT on antioxidant ability and immune responses of zebrafish (Danio rerio). Three hundred sixty healthy zebrafish were randomly grouped into four groups and exposed to different doses of TBT (0, 1, 10 and 100ngL-1). At the end of 8 weeks, the fish were sampled, and antioxidant capability, immune parameters and immune-related genes were assessed. The results showed that with an increase in TBT dose, the concentration of malonaldehyde in the liver was significantly increased (p<0.05), whereas the activities of total superoxide dismutase, catalase and glutathione peroxidase were significantly decreased (p<0.05) compared to the control. The activity and expression of lysozyme and the content of immunoglobulin M were significantly decreased compared to those of the fish exposed to 0ngL-1 TBT (p<0.05). However, the expression of the HSP70, HSP90, tumor necrosis factor-α(TNF-α), interleukins (IL-1β, IL-6), and nuclear factor-kappa B p65 (NF-κ B p65) genes were all enhanced with an increase in TBT dose. The results indicated that TBT induced oxidative stress and had immunotoxic effects on zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping