PUBLICATION
Molecular cloning and characterization of unfolded protein response genes from large yellow croaker (Larimichthys crocea) and their expression in response to dietary fatty acids
- Authors
- Liao, K., Yan, J., Li, S., Wang, T., Xu, W., Mai, K., Ai, Q.
- ID
- ZDB-PUB-160925-2
- Date
- 2017
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 203: 53-64 (Journal)
- Registered Authors
- Xu, Wei
- Keywords
- Characterization, Cloning, Dietary fatty acids, Gene expression, Large yellow croaker, Unfolded protein response
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- Dietary Fats/pharmacology*
- Fatty Acids/pharmacology*
- Fish Proteins/chemistry
- Fish Proteins/genetics
- Fish Proteins/metabolism
- Gene Expression Regulation/drug effects*
- Humans
- Perciformes/genetics*
- Perciformes/metabolism*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Unfolded Protein Response/drug effects*
- Unfolded Protein Response/genetics*
- PubMed
- 27664540 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Liao, K., Yan, J., Li, S., Wang, T., Xu, W., Mai, K., Ai, Q. (2017) Molecular cloning and characterization of unfolded protein response genes from large yellow croaker (Larimichthys crocea) and their expression in response to dietary fatty acids. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 203:53-64.
Abstract
The unfolded protein response (UPR) is a mechanism to cope with perturbed endoplasmic reticulum (ER) functions or accumulation of unfolded protein in the ER in eukaryotic cells. Furthermore, the UPR also participates in a number of physiological and pathological processes, such as nutrient sensing, lipid synthesis, and inflammatory response. In this study, four UPR-related genes (GRP78/BiP, ATF6α, XBP1 and CHO) were isolated characterized from large yellow croaker (Larimichthys crocea), and their expression in response to dietary lipid sources (various fatty acids) such as fish oil (FO), palmic acid (PA), olive oil (OO), sunflower oil (SO), and perilla oil (PO), were examined following feeding. The results showed that the four UPR-related proteins contained highly conserved functional domains and had the closest phylogenetic relationships with other fishes. Additionally, these genes were ubiquitously expressed in large yellow croaker, as in zebrafish and medaka. Moreover, GRP78, ATF6α and spliced XBP1 (XBP1s) mRNA levels in the liver, not in adipose tissue, were significantly increased in the SO group compared to the other groups (P<0.05). These results indicated that dietary SO activated UPR, and the activation of UPR might provide a mechanism to improve ER function, but probably stimulated lipid synthesis and caused inflammatory response in the liver of large yellow croaker.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping