PUBLICATION

A zebrafish mosaic assay to study mammalian stem cells in real time in vivo

Authors
Xiao, C., Qian, M., Yin, C., Zhang, Y., Hu, H., Yao, S.
ID
ZDB-PUB-160825-8
Date
2016
Source
Journal of Molecular Histology   47(5): 437-44 (Review)
Registered Authors
Yao, Shaohua
Keywords
Neural stem cell, Trans-differentiation, Transplantation, Zebrafish chimera
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Culture Techniques
  • Cell Differentiation
  • Cell Transdifferentiation
  • Endoderm/cytology
  • Mesoderm/cytology
  • Mice
  • Mosaicism*
  • Neural Stem Cells/cytology
  • Neural Stem Cells/metabolism
  • Stem Cells/cytology*
  • Stem Cells/metabolism
  • Zebrafish
PubMed
27554369 Full text @ J. Mol. Histol.
Abstract
The differentiation potentials of stem cells have been evaluated by various in vivo and in vitro assays. However, these assays have different limitations hindering efficient study of mammalian stem cells. Here we describe a rapid and powerful mosaic assay to study the differentiation potentials of stem cells in real time in vivo by using zebrafish embryo. We transplanted mouse neural stem cells into zebrafish embryos at different developmental stages and found that they mainly formed neural tissues while occasionally trans-differentiated into mesoderm- and endoderm-derived tissues. Because zebrafish embryo is transparent, the behaviors of transplanted mouse stem cells can be easily tracked in a real-time manner and at single-cell resolution. We expect that this assay may be widely applied to explore the in vivo behaviors of any stem cells available.
Errata / Notes
Erratum.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping