ZFIN ID: ZDB-PUB-160728-24
Optimizing multi-dimensional high throughput screening using zebrafish
Truong, L., Bugel, S.M., Chlebowski, A., Usenko, C.Y., Simonich, M.T., Massey Simonich, S.L., Tanguay, R.L.
Date: 2016
Source: Reproductive toxicology (Elmsford, N.Y.) 65: 139-147 (Journal)
Registered Authors: Tanguay, Robert L.
Keywords: developmental toxicity, high throughput screening, zebrafish
MeSH Terms: none
PubMed: 27453428 Full text @ Reprod. Toxicol.
ABSTRACT
The use of zebrafish for high throughput screening (HTS) for chemical bioactivity assessments is becoming routine in the fields of drug discovery and toxicology. Here we report current recommendations from our experiences in zebrafish HTS. We compared the effects of different high throughput chemical delivery methods on nominal water concentration, chemical sorption to multi-well polystyrene plates, transcription responses, and resulting whole animal responses. We demonstrate that digital dispensing consistently yields higher data quality and reproducibility compared to standard plastic tip-based liquid handling. Additionally, we illustrate the challenges in using this sensitive model for chemical assessment when test chemicals have trace impurities. Adaptation of these better practices for zebrafish HTS should increase reproducibility across laboratories.
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