PUBLICATION
Immunofluorescence Staining of Wt1 on Sections of Zebrafish Embryos and Larvae
- Authors
- Perner, B., Englert, C.
- ID
- ZDB-PUB-160716-9
- Date
- 2016
- Source
- Methods in molecular biology (Clifton, N.J.) 1467: 129-32 (Chapter)
- Registered Authors
- Englert, Christoph, Perner, Birgit
- Keywords
- Cryosectioning, Immunohistochemistry, Kidney, Pronephros, Wt1
- MeSH Terms
-
- Animals
- Cryoultramicrotomy
- Fluorescent Antibody Technique/methods*
- Kidney/metabolism
- Larva/metabolism
- Tissue Embedding
- WT1 Proteins/metabolism*
- Zebrafish/embryology*
- Zebrafish/metabolism
- Zebrafish Proteins/metabolism
- PubMed
- 27417965 Full text @ Meth. Mol. Biol.
Citation
Perner, B., Englert, C. (2016) Immunofluorescence Staining of Wt1 on Sections of Zebrafish Embryos and Larvae. Methods in molecular biology (Clifton, N.J.). 1467:129-32.
Abstract
Immunohistochemistry is one of the most powerful tools for direct visualization of distribution and localization of gene products. The presented protocol provides an opportunity to determine the localization patterns of Wt1 in zebrafish via antibody staining.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping