PUBLICATION

Immunofluorescence Staining of Wt1 on Sections of Zebrafish Embryos and Larvae

Authors
Perner, B., Englert, C.
ID
ZDB-PUB-160716-9
Date
2016
Source
Methods in molecular biology (Clifton, N.J.)   1467: 129-32 (Chapter)
Registered Authors
Englert, Christoph, Perner, Birgit
Keywords
Cryosectioning, Immunohistochemistry, Kidney, Pronephros, Wt1
MeSH Terms
  • Animals
  • Cryoultramicrotomy
  • Fluorescent Antibody Technique/methods*
  • Kidney/metabolism
  • Larva/metabolism
  • Tissue Embedding
  • WT1 Proteins/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/metabolism
PubMed
27417965 Full text @ Meth. Mol. Biol.
Abstract
Immunohistochemistry is one of the most powerful tools for direct visualization of distribution and localization of gene products. The presented protocol provides an opportunity to determine the localization patterns of Wt1 in zebrafish via antibody staining.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping