PUBLICATION
Strategies for analyzing cardiac phenotypes in the zebrafish embryo
- Authors
- Houk, A.R., Yelon, D.
- ID
- ZDB-PUB-160618-14
- Date
- 2016
- Source
- Methods in cell biology 134: 335-68 (Chapter)
- Registered Authors
- Yelon, Deborah
- Keywords
- Atrioventricular canal, Blood flow, Cardiac chamber formation, Cardiac conduction, Cardiac contractility, Cardiac morphogenesis, Cardiac specification, Heart development, Myocardial differentiation, Trabeculation
- MeSH Terms
-
- Animals
- Embryonic Development/genetics*
- Gene Expression Regulation, Developmental
- Heart/growth & development*
- Morphogenesis/genetics*
- Morpholinos/genetics
- Mutation
- Phenotype
- Zebrafish/genetics
- Zebrafish/growth & development*
- PubMed
- 27312497 Full text @ Meth. Cell. Biol.
Citation
Houk, A.R., Yelon, D. (2016) Strategies for analyzing cardiac phenotypes in the zebrafish embryo. Methods in cell biology. 134:335-68.
Abstract
The molecular mechanisms underlying cardiogenesis are of critical biomedical importance due to the high prevalence of cardiac birth defects. Over the past two decades, the zebrafish has served as a powerful model organism for investigating heart development, facilitated by its powerful combination of optical access to the embryonic heart and plentiful opportunities for genetic analysis. Work in zebrafish has identified numerous factors that are required for various aspects of heart formation, including the specification and differentiation of cardiac progenitor cells, the morphogenesis of the heart tube, cardiac chambers, and atrioventricular canal, and the establishment of proper cardiac function. However, our current roster of regulators of cardiogenesis is by no means complete. It is therefore valuable for ongoing studies to continue pursuit of additional genes and pathways that control the size, shape, and function of the zebrafish heart. An extensive arsenal of techniques is available to distinguish whether particular mutations, morpholinos, or small molecules disrupt specific processes during heart development. In this chapter, we provide a guide to the experimental strategies that are especially effective for the characterization of cardiac phenotypes in the zebrafish embryo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping