PUBLICATION
Longitudinal Effects of Embryonic Exposure to Cocaine on Morphology, Cardiovascular Physiology, and Behavior in Zebrafish
- Authors
- Mersereau, E.J., Boyle, C.A., Poitra, S., Espinoza, A., Seiler, J., Longie, R., Delvo, L., Szarkowski, M., Maliske, J., Chalmers, S., Darland, D.C., Darland, T.
- ID
- ZDB-PUB-160604-4
- Date
- 2016
- Source
- International Journal of Molecular Sciences 17(6): (Journal)
- Registered Authors
- Darland, Tristan
- Keywords
- behavior, cardiovascular physiology, cocaine, zebrafish
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects*
- Cardiovascular Physiological Phenomena/drug effects*
- Cocaine/toxicity*
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian/drug effects
- Zebrafish/embryology*
- PubMed
- 27258254 Full text @ Int. J. Mol. Sci.
Citation
Mersereau, E.J., Boyle, C.A., Poitra, S., Espinoza, A., Seiler, J., Longie, R., Delvo, L., Szarkowski, M., Maliske, J., Chalmers, S., Darland, D.C., Darland, T. (2016) Longitudinal Effects of Embryonic Exposure to Cocaine on Morphology, Cardiovascular Physiology, and Behavior in Zebrafish. International Journal of Molecular Sciences. 17(6).
Abstract
A sizeable portion of the societal drain from cocaine abuse results from the complications of in utero drug exposure. Because of challenges in using humans and mammalian model organisms as test subjects, much debate remains about the impact of in utero cocaine exposure. Zebrafish offer a number of advantages as a model in longitudinal toxicology studies and are quite sensitive physiologically and behaviorally to cocaine. In this study, we have used zebrafish to model the effects of embryonic pre-exposure to cocaine on development and on subsequent cardiovascular physiology and cocaine-induced conditioned place preference (CPP) in longitudinal adults. Larval fish showed a progressive decrease in telencephalic size with increased doses of cocaine. These treated larvae also showed a dose dependent response in heart rate that persisted 24 h after drug cessation. Embryonic cocaine exposure had little effect on overall health of longitudinal adults, but subtle changes in cardiovascular physiology were seen including decreased sensitivity to isoproterenol and increased sensitivity to cocaine. These longitudinal adult fish also showed an embryonic dose-dependent change in CPP behavior, suggesting an increased sensitivity. These studies clearly show that pre-exposure during embryonic development affects subsequent cocaine sensitivity in longitudinal adults.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping