PUBLICATION
Imaging Cancer Angiogenesis and Metastasis in a Zebrafish Embryo Model
- Authors
- Tulotta, C., He, S., van der Ent, W., Chen, L., Groenewoud, A., Spaink, H.P., Snaar-Jagalska, B.E.
- ID
- ZDB-PUB-160512-21
- Date
- 2016
- Source
- Advances in experimental medicine and biology 916: 239-63 (Chapter)
- Registered Authors
- Snaar-Jagalska, Ewa B., Spaink, Herman P., van der Ent, Wietske
- Keywords
- Angiogenesis, Extravasation, In vivo imaging, Metastasis, Tumor inflammation
- MeSH Terms
-
- Animals
- Disease Models, Animal*
- Fluorescent Dyes
- Heterografts
- Neoplasm Metastasis
- Neoplasms/blood supply*
- Neoplasms/pathology
- Neovascularization, Pathologic*
- Zebrafish/embryology*
- PubMed
- 27165357 Full text @ Adv. Exp. Med. Biol.
Citation
Tulotta, C., He, S., van der Ent, W., Chen, L., Groenewoud, A., Spaink, H.P., Snaar-Jagalska, B.E. (2016) Imaging Cancer Angiogenesis and Metastasis in a Zebrafish Embryo Model. Advances in experimental medicine and biology. 916:239-63.
Abstract
Tumor angiogenesis and metastasis are key steps of cancer progression. In vitro and animal model studies have contributed to partially elucidating the mechanisms involved in these processes and in developing therapies. Besides the improvements in fundamental research and the optimization of therapeutic regimes, cancer still remains a major health threatening condition and therefore the development of new models is needed. The zebrafish is a powerful tool to study tumor angiogenesis and metastasis, because it allows the visualization of fluorescently labelled tumor cells inducing vessel remodeling, disseminating and invading surrounding tissues in a whole transparent embryo. The embryo model has also been used to address the contribution of the tumor stroma in sustaining tumor angiogenesis and spreading. Simultaneously, new anti-angiogenic drugs and compounds affecting malignant cell survival and migration can be tested by simply adding the compound into the water of living embryos. Therefore the zebrafish model offers the opportunity to gain more knowledge on cancer angiogenesis and metastasis in vivo with the final aim of providing new translational insights into therapeutic approaches to help patients.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping