PUBLICATION
Identification and functional characterization of fish-egg lectin in zebrafish
- Authors
- Wang, Y., Bu, L., Yang, L., Li, H., Zhang, S.
- ID
- ZDB-PUB-160316-5
- Date
- 2016
- Source
- Fish & shellfish immunology 52: 23-30 (Journal)
- Registered Authors
- Li, Hongyan
- Keywords
- Fish-egg lectin, Maternal immunity, Opsonization, Phagocytosis, Zebrafish
- MeSH Terms
-
- Aeromonas hydrophila/physiology
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- DNA, Complementary/genetics
- DNA, Complementary/metabolism
- Embryo, Nonmammalian/immunology
- Fish Proteins/genetics
- Fish Proteins/isolation & purification
- Fish Proteins/metabolism*
- Fish Proteins/pharmacology
- Gram-Negative Bacteria/physiology
- Gram-Positive Bacteria/physiology
- Immunity, Innate/drug effects
- Immunity, Innate/immunology*
- Lectins/genetics
- Lectins/isolation & purification
- Lectins/metabolism*
- Lectins/pharmacology
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Zebrafish/genetics*
- Zebrafish/immunology*
- Zebrafish/metabolism
- Zebrafish/microbiology
- PubMed
- 26975412 Full text @ Fish Shellfish Immunol.
Citation
Wang, Y., Bu, L., Yang, L., Li, H., Zhang, S. (2016) Identification and functional characterization of fish-egg lectin in zebrafish. Fish & shellfish immunology. 52:23-30.
Abstract
Fish-egg lectins (FELs) are identified in several species of fishes, but their activity and mode of action remain largely unknown in early life stages. Here we showed that zebrafish FEL (zFEL) was a maternal factor, which was capable of interacting with Gram-negative and Gram-positive bacteria and enhancing the phagocytosis of the bacteria by macrophages. Interestingly, microinjection of purified native zFEL into the embryos (resulting in the increase of zFEL in the embryos) markedly promoted the resistance of the embryos to the pathogenic Aeromonas hydrophila. Taken together, zFEL appears a maternal immune-relevant molecule capable of defending the developing embryos/larvae from pathogenic attacks.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping