PUBLICATION

Spinal motor neurons are regenerated after mechanical lesion and genetic ablation in larval zebrafish

Authors
Ohnmacht, J., Yang, Y.J., Maurer, G.W., Barreiro-Iglesias, A., Tsarouchas, T.M., Wehner, D., Sieger, D., Becker, C.G., Becker, T.
ID
ZDB-PUB-160312-9
Date
2016
Source
Development (Cambridge, England)   143(9): 1464-74 (Journal)
Registered Authors
Barreiro-Iglesias, Antón, Becker, Catherina G., Becker, Thomas, Ohnmacht, Jochen, Sieger, Dirk, Tsarouchas, Themistoklis, Wehner, Daniel, Yang, Yujie
Keywords
Dopamine, Macrophage, Microglia, Nitroreductase, Hb9, Olig2, Sox10
MeSH Terms
  • Animals
  • Dexamethasone/pharmacology
  • Immunity, Innate/drug effects
  • Immunosuppressive Agents/pharmacology
  • Larva/cytology*
  • Larva/genetics
  • Macrophages/immunology
  • Metronidazole/pharmacology
  • Microglia/metabolism
  • Motor Neurons/cytology*
  • Nerve Regeneration/drug effects
  • Nerve Regeneration/physiology*
  • Neural Stem Cells/cytology*
  • Oligodendroglia/cytology
  • PAX2 Transcription Factor/metabolism
  • Spinal Cord/cytology*
  • Spinal Cord Injuries/metabolism*
  • Zebrafish/growth & development*
  • Zebrafish Proteins/metabolism
PubMed
26965370 Full text @ Development
Abstract
In adult zebrafish, relatively quiescent progenitor cells show lesion-induced generation of motor neurons. Developmental motor neuron generation from the spinal motor neuron progenitor domain (pMN) sharply declines already at 48 hours post-fertilisation (hpf). After that, mostly oligodendrocytes are generated from the same domain. We demonstrate here that within 48 hours after a spinal lesion or specific genetic ablation of motor neurons at 72 hpf, the pMN domain reverts to motor neuron generation at the expense of oligodendrogenesis. In contrast, generation of dorsal Pax2-positive interneurons was not altered. Larval motor neuron regeneration can be boosted by dopaminergic drugs, similar to adult regeneration. We use larval lesions to show that pharmacological suppression of the cellular response of the innate immune system inhibits motor neuron regeneration. Hence, we have established a rapid larval regeneration paradigm. Both, mechanical lesion or motor neuron ablation are sufficient to reveal a high degree of developmental flexibility of pMN progenitor cells. In addition, we show an important influence of the immune system on motor neuron regeneration from these progenitor cells.
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