PUBLICATION
Alcohol-induced behavioral changes in zebrafish: The role of dopamine D2-like receptors
- Authors
- Tran, S., Facciol, A., Gerlai, R.
- ID
- ZDB-PUB-160310-9
- Date
- 2016
- Source
- Psychopharmacology 233(11): 2119-28 (Journal)
- Registered Authors
- Gerlai, Robert T.
- Keywords
- Alcohol, Anxiety, Haloperidol, Locomotor activity, Zebrafish
- MeSH Terms
-
- Animals
- Anti-Anxiety Agents/pharmacology
- Anxiety/chemically induced
- Anxiety/psychology
- Behavior, Animal/drug effects*
- Central Nervous System Depressants/antagonists & inhibitors
- Central Nervous System Depressants/pharmacology*
- Dopamine Antagonists/pharmacology
- Dose-Response Relationship, Drug
- Ethanol/antagonists & inhibitors
- Ethanol/pharmacology*
- Haloperidol/antagonists & inhibitors
- Haloperidol/pharmacology
- Locomotion/drug effects
- Motor Activity/drug effects
- Receptors, Dopamine D2/drug effects*
- Zebrafish/physiology*
- PubMed
- 26955840 Full text @ Psychpharma
Citation
Tran, S., Facciol, A., Gerlai, R. (2016) Alcohol-induced behavioral changes in zebrafish: The role of dopamine D2-like receptors. Psychopharmacology. 233(11):2119-28.
Abstract
Rationale The dopaminergic system has been proposed to mediate alcohol-induced locomotor activity, yet the mechanisms underlying this behavioral response remain poorly understood.
Objectives This study was conducted to investigate the role of dopamine D2-like receptors in mediating alcohol-induced behavioral responses.
Methods In experiment 1, we examined the effects of high concentrations (0, 2.5, 5, 10 μM) of haloperidol on motor responses. In experiment 2, we examined the effects of low concentrations (0, 0.625, 1.25, 2.5 μM) of haloperidol on anxiety-like behavioral responses using the novel tank test. In experiment 3, we examined the effect of pre-treating zebrafish with different concentrations of haloperidol (0, 0.625, 2.5 μM) and subsequently exposing them to 0 or 1 % alcohol.
Results In experiment 1, haloperidol induced an inverted U-shaped concentration-dependent increase in locomotor activity. In experiment 2, haloperidol (2.5 μM) reduced the absolute turn angle and freezing behavior in a new environment. In experiment 3, acute alcohol exposure significantly increased locomotor activity and decreased anxiety-like behavioral responses. Pre-treating zebrafish with the lower dose of haloperidol (0.625 μM) abolished the alcohol-induced locomotor activity, without altering anxiety-like behavioral responses. However, pre-treating with the higher dose of haloperidol (2.5 μM) abolished both alcohol-induced increase of locomotor activity and reduction of anxiety-like behavioral responses.
Conclusion The results suggest alcohol-induced locomotor hyperactivity in zebrafish is mediated via activation of dopamine D2-like receptors, whereas anxiety-like behavioral responses may only be altered by a high haloperidol concentration, at which dose the drug may affect receptors other than D2-R.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping