PUBLICATION
ECM couples the convergence movements of mesoderm and neural plate during the early stages of neurulation
- Authors
- Araya, C., Carmona-Fontaine, C., Clarke, J.D.
- ID
- ZDB-PUB-160305-24
- Date
- 2016
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 245(5): 580-9 (Journal)
- Registered Authors
- Araya Garcia, Claudio, Clarke, Jon
- Keywords
- Zebrafish, extracellular matrix, morphogenesis, neurulation
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Extracellular Matrix/physiology*
- Fibronectins/physiology
- Laminin/physiology
- Mesoderm/metabolism*
- Morphogenesis
- Neural Plate/metabolism*
- Neural Tube
- Neurulation*
- Zebrafish
- PubMed
- 26933766 Full text @ Dev. Dyn.
Citation
Araya, C., Carmona-Fontaine, C., Clarke, J.D. (2016) ECM couples the convergence movements of mesoderm and neural plate during the early stages of neurulation. Developmental Dynamics : an official publication of the American Association of Anatomists. 245(5):580-9.
Abstract
Background During the initial stages zebrafish neurulation, neural plate cells undergo highly coordinated movements before they assemble into a multi-cellular solid neural rod. We have previously identified that the underlying mesoderm is critical to ensure such coordination and generate correct neural tube organization. However, how inter-tissue co-ordination is achieved in vivo during zebrafish neural tube morphogenesis is unknown.
Results In this work, we use quantitative live imaging to study the co-ordinated movements of neural ectoderm and mesoderm during dorsal tissue convergence. We show the extracellular matrix components Laminin and Fibronectin that lie between mesoderm and neural plate act to couple the movements of neural plate and mesoderm during early stages of neurulation and to maintain the close apposition of these two tissues.
Conclusions Our study highlights the importance of the ECM proteins Laminin and Fibronectin in coupling the movements and spatial proximity of mesoderm and neuroectoderm during the morphogenetic movements of neurulation. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping