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ZFIN ID: ZDB-PUB-160214-2
The zebrafish fast myosin light chain mylpfa:H2B-GFP transgene is a useful tool for in vivo imaging of myocyte fusion in the vertebrate embryo
Zhang, W., Roy, S.
Date: 2016
Source: Gene expression patterns : GEP 20(2): 106-10 (Journal)
Registered Authors: Roy, Sudipto
Keywords: Zebrafish myocyte fusion, genetic screen, in vivo imaging, mylpfa:H2B-GFP transgene
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation/genetics*
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins/genetics
  • Histones/genetics
  • Muscle Development/genetics*
  • Muscle Fibers, Skeletal/metabolism
  • Myosin Light Chains/genetics*
  • Promoter Regions, Genetic
  • Receptors, Cell Surface/genetics
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish Proteins/genetics
PubMed: 26872916 Full text @ Gene Expr. Patterns
FIGURES
ABSTRACT
Skeletal muscle fibers are multinucleated syncytia that arise from the fusion of mononucleated precursors, the myocytes, during embryonic development, muscle hypertrophy in post-embryonic growth and muscle regeneration after injury. Even though myocyte fusion is central to skeletal muscle differentiation, our current knowledge of the molecular mechanism of myocyte fusion in the vertebrates is rather limited. Previous work, from our group and others, has shown that the zebrafish embryo is a very useful model for investigating the cell biology and genetics of vertebrate myocyte fusion in vivo.
Here, we report the generation of a stable transgenic zebrafish strain that expresses the Histone 2B-GFP (H2B-GFP) fusion protein in the nuclei of all fast-twitch muscle fibers under the control of the fast-twitch muscle-specific myosin light chain, phosphorylatable, fast skeletal muscle a (mylpfa) gene promoter. By introducing this transgene into a mutant for junctional adhesion molecule 3b (jam3b), which encodes a cell adhesion protein previously implicated in myocyte fusion, we demonstrate the feasibility of using this transgene for the analysis of myocyte fusion during the differentiation of the trunk musculature of the zebrafish embryo.
Since we know so little about the molecules regulating vertebrate myocyte fusion, we propose that the mylpfa:H2B-GFP transgene will be a very useful reporter for conducting forward and reverse genetic screens to identify new components regulating vertebrate myocyte fusion.
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