PUBLICATION

Experimental Approaches to Study Endothelial Responses to Shear Stress.

Authors
Bowden, N., Bryan, M., Duckles, H., Feng, S., Hsiao, S., Kim, R., Mahmoud, M., Moers, B., Serbanovic-Canic, J., Ioannis, I., Ridger, V., Evans, P.
ID
ZDB-PUB-160117-8
Date
2016
Source
Antioxidants & redox signaling   25(7): 389-400 (Review)
Registered Authors
Kim, Rosemary
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Endothelial Cells/physiology*
  • Endothelium, Vascular/physiology*
  • Humans
  • In Vitro Techniques
  • Mechanotransduction, Cellular*
  • Stress, Mechanical*
PubMed
26772071 Full text @ Antioxid. Redox Signal.
Abstract
Shear stress controls multiple physiological processes in endothelial cells. This article describes some of the experimental techniques that can be used to study endothelial responses to shear stress. It includes an appraisal of large animal, rodent and zebrafish models of vascular mechanoresponsiveness. It also describes several bioreactors to apply flow to cells, and physical methods to separate mechanoresponses from mass transport mechanisms. We conclude that combining in vitro and in vivo approaches can provide a detailed mechanistic view of vascular responses to force, and that high throughput systems are required for unbiased assessment of the function of shear-induced molecules.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping