PUBLICATION
Investigation of the Effects of Perfluorooctanoic Acid (PFOA) and Perfluorooctane Sulfonate (PFOS) on Apoptosis and Cell Cycle in a Zebrafish (Danio rerio) Liver Cell Line
- Authors
- Cui, Y., Liu, W., Xie, W., Yu, W., Wang, C., Chen, H.
- ID
- ZDB-PUB-151223-6
- Date
- 2015
- Source
- International Journal of Environmental Research and Public Health 12: 15673-82 (Journal)
- Registered Authors
- Keywords
- apoptosis, perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), zebrafish liver cells (ZFL)
- MeSH Terms
-
- Alkanesulfonic Acids/toxicity*
- Animals
- Apoptosis/drug effects*
- Caprylates/toxicity*
- Cell Cycle/drug effects*
- Cell Line
- Fluorocarbons/toxicity*
- Hepatocytes/drug effects*
- Liver/drug effects*
- Toxicity Tests
- Zebrafish/physiology*
- PubMed
- 26690195 Full text @ Int. J. Environ. Res. Public Health
Citation
Cui, Y., Liu, W., Xie, W., Yu, W., Wang, C., Chen, H. (2015) Investigation of the Effects of Perfluorooctanoic Acid (PFOA) and Perfluorooctane Sulfonate (PFOS) on Apoptosis and Cell Cycle in a Zebrafish (Danio rerio) Liver Cell Line. International Journal of Environmental Research and Public Health. 12:15673-82.
Abstract
This study aimed to explore the effects of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) on apoptosis and cell cycle in a zebrafish (Danio rerio) liver cell line (ZFL). Treatment groups included a control group, PFOA-IC50, PFOA-IC80, PFOS-IC50 and PFOS-IC80 groups. IC50 and IC80 concentrations were identified by cellular modeling and MTT assays. mRNA levels of p53, Bcl-2, Bax, Caspase-3 and NF-κB p65 were detected by qPCR. Cell apoptosis and cell cycle were detected by flow cytometry and the protein levels of p53, Bcl-2, Bax, Caspase-3 and NF-κB p65 were determined by western blotting. Both PFOA and PFOS inhibited the growth of zebrafish liver cells, and the inhibition rate of PFOS was higher than that of PFOA. Bcl-2 expression levels in the four groups were significantly higher than the control group and Bcl-2 increased significantly in the PFOA-IC80 group. However, the expression levels of Bax in the four treatment groups were higher than the control group. The percentage of cell apoptosis increased significantly with the treatment of PFOA and PFOS (p < 0.05). Cell cycle and cell proliferation were blocked in both the PFOA-IC80 and PFOS-IC80 groups, indicating that PFOA-IC80 and PFOS-IC50 enhanced apoptosis in ZFL cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping