ZFIN ID: ZDB-PUB-151216-29
Genome editing in zebrafish: a practical overview
Sertori, R., Trengove, M., Basheer, F., Ward, A.C., Liongue, C.
Date: 2016
Source: Briefings in Functional Genomics   15(4): 322-30 (Review)
Registered Authors: Liongue, Clifford, Trengove, Monique, Ward, Alister C.
Keywords: CRISPR, HDR, TALENs, genome editing, zebrafish, zinc finger nucleases
MeSH Terms:
  • Animals
  • CRISPR-Cas Systems*
  • Gene Editing/methods*
  • Genome
  • Zebrafish/genetics*
PubMed: 26654901 Full text @ Brief. Funct. Genomics
Zebrafish is a powerful model for the study of vertebrate development, being amenable to a wide range of genetic and other manipulations to probe the molecular basis of development and its perturbation in disease. Over recent years, genome editing approaches have become increasingly used as an efficient and sophisticated approach to precisely engineer the zebrafish genome, which has further enhanced the utility of this organism. This review provides a practical overview of genome editing and its application in zebrafish research, including alternate strategies for introducing and screening for specific genetic changes.