PUBLICATION
Molecular characterization and expression analyses of cDNAs encoding the thioredoxin-interacting protein and selenoprotein P genes and histological changes in Nile tilapia (Oreochromis niloticus) in response to silver nanoparticle exposure
- Authors
- Thummabancha, K., Onparn, N., Srisapoome, P.
- ID
- ZDB-PUB-151202-4
- Date
- 2016
- Source
- Gene 577(2): 161-73 (Journal)
- Registered Authors
- Keywords
- Gene expression, Histopathology, Nile tilapia, Selenoprotein P, Silver nanoparticles, Thioredoxin-interacting protein
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Carrier Proteins/genetics*
- Carrier Proteins/metabolism
- Conserved Sequence
- Down-Regulation
- Fish Proteins/genetics*
- Fish Proteins/metabolism
- Liver/cytology
- Liver/drug effects
- Liver/metabolism*
- Metal Nanoparticles/adverse effects*
- Metal Nanoparticles/chemistry
- Molecular Sequence Data
- Organ Specificity
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Selenoproteins/genetics*
- Selenoproteins/metabolism
- Silver/pharmacology*
- Thioredoxins/metabolism
- Tilapia
- PubMed
- 26621381 Full text @ Gene
Citation
Thummabancha, K., Onparn, N., Srisapoome, P. (2016) Molecular characterization and expression analyses of cDNAs encoding the thioredoxin-interacting protein and selenoprotein P genes and histological changes in Nile tilapia (Oreochromis niloticus) in response to silver nanoparticle exposure. Gene. 577(2):161-73.
Abstract
Herein, Nile tilapia thioredoxin-interacting protein (On-TXNIP) and selenoprotein P (On-SEPP) cDNAs were cloned and characterized. The full-length On-TXNIP cDNA contained 2 arrestin domains, 2 conserved cysteine residues that bind to thioredoxin to inhibit thioredoxin function, and 2 PPXY motifs, which negatively regulate the protein by stimulating binding to E3 ubiquitin ligase. The On-SEPP cDNA contained 17 selenocysteines (Sec) encoded by the TGA codon, which can be recognized as either a stop codon or a Sec codon. The On-SEPP cDNA also carried 2 typical SECIS elements located in the 3'UTR that are important for selenocysteine translation. Evolutionary analyses of both the On-TXNIP and On-SEPP genes revealed that these genes are closely related to the TXNIP and SEPP genes in zebrafish (Danio rerio), with amino acid similarities of 91.8% and 61.9%, respectively. A normal tissue distribution analysis indicated that the On-TXNIP and On-SEPP genes were ubiquitously expressed in all tissues examined, and the highest expression levels of these genes were observed in peripheral blood leukocytes (PBLs) and the trunk kidney, respectively. The expression levels of On-TXNIP and On-SEPP transcripts were acutely and chronically analyzed following the injection of fish with 1, 10 or 100mg/kg silver nanoparticles (Ag NPs). Significant up-regulation of On-TXNIP and On-SEPP transcripts was observed in the liver, spleen, and head kidney at the early phase of Ag NP exposure (hours 6 through 48). Down-regulation of On-SEPP transcripts was clearly observed in the liver at weeks 1 to 4. Histopathology analysis demonstrated that the fish livers exhibited a dramatic infiltration of Kupffer cells, elevated bi-nucleated cells, expanded sinusoidal blood congestion and severe necrosis in a dose-dependent manner. Based on these findings, coupling of the expression analysis of these two cellular stress response genes and histopathological observation of fish exposed to Ag NPs should be reliable for the assessment of Ag NP contamination in teleost fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping