PUBLICATION
A microfluidic device to study neuronal and motor responses to acute chemical stimuli in zebrafish
- Authors
- Candelier, R., Sriti Murmu, M., Alejo Romano, S., Jouary, A., Debrégeas, G., Sumbre, G.
- ID
- ZDB-PUB-150722-5
- Date
- 2015
- Source
- Scientific Reports 5: 12196 (Journal)
- Registered Authors
- Sumbre, Germán
- Keywords
- Gustatory system, Sensorimotor processing
- MeSH Terms
-
- Animals
- Behavior, Animal
- Lab-On-A-Chip Devices*
- Motor Activity/physiology*
- Neurons/physiology*
- Olfactory Bulb/physiology
- Rheology
- Smell/physiology
- Taste/physiology
- Zebrafish/physiology*
- PubMed
- 26194888 Full text @ Sci. Rep.
Citation
Candelier, R., Sriti Murmu, M., Alejo Romano, S., Jouary, A., Debrégeas, G., Sumbre, G. (2015) A microfluidic device to study neuronal and motor responses to acute chemical stimuli in zebrafish. Scientific Reports. 5:12196.
Abstract
Zebrafish larva is a unique model for whole-brain functional imaging and to study sensory-motor integration in the vertebrate brain. To take full advantage of this system, one needs to design sensory environments that can mimic the complex spatiotemporal stimulus patterns experienced by the animal in natural conditions. We report on a novel open-ended microfluidic device that delivers pulses of chemical stimuli to agarose-restrained larvae with near-millisecond switching rate and unprecedented spatial and concentration accuracy and reproducibility. In combination with two-photon calcium imaging and recordings of tail movements, we found that stimuli of opposite hedonic values induced different circuit activity patterns. Moreover, by precisely controlling the duration of the stimulus (50-500 ms), we found that the probability of generating a gustatory-induced behavior is encoded by the number of neurons activated. This device may open new ways to dissect the neural-circuit principles underlying chemosensory perception.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping