ZFIN ID: ZDB-PUB-150708-2
Generation of Late-Born Neurons in the Ventral Spinal Cord requires the coordination of Retinoic Acid and Notch signaling
Ryu, J.H., Kong, H.J., Park, J.Y., Lim, K.E., An, C.M., Lee, J., Yeo, S.Y.
Date: 2015
Source: Neuroscience letters   602: 95-8 (Journal)
Registered Authors: Yeo, Sang-Yeob
Keywords: Kolmer-Agduhr neuron, citral, mindbomb, retinoic acid, transgenic zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation
  • Embryo, Nonmammalian
  • GABAergic Neurons/cytology
  • GABAergic Neurons/metabolism
  • HSP70 Heat-Shock Proteins/genetics
  • Interneurons/cytology
  • Interneurons/metabolism
  • Motor Neurons/cytology
  • Motor Neurons/metabolism
  • Neural Stem Cells/cytology
  • Neural Stem Cells/metabolism
  • Neurogenesis
  • Neurons/cytology*
  • Neurons/metabolism
  • Promoter Regions, Genetic
  • Receptors, Notch/physiology*
  • Sensory Receptor Cells/cytology
  • Sensory Receptor Cells/metabolism
  • Signal Transduction
  • Spinal Cord/cytology*
  • Spinal Cord/embryology
  • Spinal Cord/metabolism
  • Tretinoin/pharmacology
  • Tretinoin/physiology*
  • Ubiquitin-Protein Ligases/genetics
  • Ubiquitin-Protein Ligases/physiology
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology
PubMed: 26151587 Full text @ Neurosci. Lett.
Neural progenitor cells generate various types of neurons and glia in a tightly regulated manner. During primary neurogenesis, retinoic acid (RA) acts earlier than Notch signaling and regulates differentiation and proliferation by upregulating proneural and neurogenic genes in the neural plate. However, the relationship between Notch signaling and the retinoid pathway during late neurogenesis remains unclear. We investigated the role of Mindbomb (Mib)-mediated Notch signaling in the differentiation of neural progenitors during late neurogenesis by overexpressing Mib and administering RA to Tg[hsp70-Mib:EGFP]. The majority of cells in the p3 domain differentiated into GABAergic Kolmer-Agduhr (KA) cells in Tg[hsp70-mib:EGFP]embryos heat-shocked during late neurogenesis, whereas these phenotypes were suppressed by exogenous RA. Our observations suggest that Mib-mediated Notch signaling plays a critical role in the temporal differentiation of neural progenitors, and that the generation of late-born KA" cells is regulated by the interplay between Mib and RA.