PUBLICATION
Short-term exposure of arsenite disrupted thyroid endocrine system and altered gene transcription in the HPT axis in zebrafish
- Authors
- Sun, H.J., Li, H.B., Xiang, P., Zhang, X., Ma, L.Q.
- ID
- ZDB-PUB-150610-2
- Date
- 2015
- Source
- Environmental pollution (Barking, Essex : 1987) 205: 145-152 (Journal)
- Registered Authors
- Keywords
- Arsenite, Gene transcription, Hypothalamic–pituitary–thyroid axis, Oxidative stress, Thyroid endocrine, Zebrafish
- MeSH Terms
-
- Animals
- Arsenites/toxicity*
- Gene Expression/drug effects
- Hypothalamus/drug effects*
- Hypothalamus/metabolism
- Liver/drug effects
- Liver/metabolism
- Pituitary Gland/drug effects*
- Pituitary Gland/metabolism
- Thyroid Gland/drug effects*
- Thyroid Gland/metabolism
- Thyroid Hormones/metabolism*
- Transcription, Genetic/drug effects
- Water Pollutants/toxicity
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 26057477 Full text @ Environ. Pollut.
- CTD
- 26057477
Citation
Sun, H.J., Li, H.B., Xiang, P., Zhang, X., Ma, L.Q. (2015) Short-term exposure of arsenite disrupted thyroid endocrine system and altered gene transcription in the HPT axis in zebrafish. Environmental pollution (Barking, Essex : 1987). 205:145-152.
Abstract
Arsenic (As) pollution in aquatic environment may adversely impact fish health by disrupting their thyroid hormone homeostasis. In this study, we explored the effect of short-term exposure of arsenite (AsIII) on thyroid endocrine system in zebrafish. We measured As concentrations, As speciation, and thyroid hormone thyroxine levels in whole zebrafish, oxidative stress (H2O2) and damage (MDA) in the liver, and gene transcription in hypothalamic-pituitary-thyroid (HPT) axis in the brain and liver tissues of zebrafish after exposing to different AsIII concentrations for 48 h. Result indicated that exposure to AsIII increased inorganic As in zebrafish to 0.46-0.72 mg kg(-1), induced oxidative stress with H2O2 being increased by 1.4-2.5 times and caused oxidative damage with MDA being augmented by 1.6 times. AsIII exposure increased thyroxine levels by 1.3-1.4 times and modulated gene transcription in HPT axis. Our study showed AsIII caused oxidative damage, affected thyroid endocrine system and altered gene transcription in HPT axis in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping