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ZFIN ID: ZDB-PUB-150601-1
Pharmacological study of the light/dark preference test in zebrafish (Danio rerio): Waterborne administration
Magno, L.D., Fontes, A., Gonçalves, B.M., Gouveia, A.
Date: 2015
Source: Pharmacology, biochemistry, and behavior   135: 169-76 (Journal)
Registered Authors:
Keywords: Animal model, Anxiety, Behavior, Drugs, Zebrafish
MeSH Terms:
  • Animals
  • Anti-Anxiety Agents/administration & dosage*
  • Anti-Anxiety Agents/pharmacology*
  • Anxiety/drug therapy
  • Anxiety/psychology*
  • Behavior, Animal/drug effects*
  • Darkness*
  • Dose-Response Relationship, Drug
  • Light*
  • Motor Activity/drug effects
  • Water
  • Zebrafish
PubMed: 26026898 Full text @ Pharmacol. Biochem. Behav.
Anxiety is a complex disorder; thus, its mechanisms remain unclear. Zebrafish (Danio rerio) are a promising pharmacological model for anxiety research. Light/dark preference test is a behaviorally validated measure of anxiety in zebrafish; however, it requires pharmacological validation. We sought to evaluate the sensitivity of the light/dark preference test in adult zebrafish by immersing them in drug solutions containing clonazepam, buspirone, imipramine, fluoxetine, paroxetine, haloperidol, risperidone, propranolol, or ethanol. The time spent in the dark environment, the latency time to first crossing, and the number of midline crossings were analyzed. Intermediate concentrations of clonazepam administered for 600s decreased the time spent in the dark and increased locomotor activity. Buspirone reduced motor activity. Imipramine and fluoxetine increased time spent in the dark and the first latency, and decreased the number of alternations. Paroxetine did not alter the time in the dark; however, it increased the first latency time and decreased locomotor activity. Haloperidol decreased the time spent in the dark at low concentrations. Risperidone and propranolol did not change any parameters. Ethanol reduced the time spent in the dark and increased the number of crossings at intermediate concentrations. These results corroborate previous work using intraperitoneal drug administration in zebrafish and rodents, suggesting that water drug delivery in zebrafish can effectively be used as an animal anxiety model.