PUBLICATION
Genome-wide identification, evolution and expression analysis of nuclear receptor superfamily in Nile tilapia, Oreochromis niloticus
- Authors
- Cheng, Y.Y., Tao, W.J., Chen, J.L., Sun, L.N., Zhou, L.Y., Song, Q., Wang, D.S.
- ID
- ZDB-PUB-150531-4
- Date
- 2015
- Source
- Gene 569(1): 141-52 (Journal)
- Registered Authors
- Keywords
- Nuclear receptor superfamily, gonadal expression profile, phylogenetic analysis, tilapia, tissue distribution
- MeSH Terms
-
- Animals
- Cichlids/genetics*
- Evolution, Molecular*
- Gene Expression Profiling
- Gene Expression Regulation
- Genome
- Humans
- Multigene Family/genetics
- Receptors, Cytoplasmic and Nuclear/biosynthesis
- Receptors, Cytoplasmic and Nuclear/genetics*
- Receptors, Cytoplasmic and Nuclear/isolation & purification
- Sequence Alignment
- Tissue Distribution
- PubMed
- 26024593 Full text @ Gene
Citation
Cheng, Y.Y., Tao, W.J., Chen, J.L., Sun, L.N., Zhou, L.Y., Song, Q., Wang, D.S. (2015) Genome-wide identification, evolution and expression analysis of nuclear receptor superfamily in Nile tilapia, Oreochromis niloticus. Gene. 569(1):141-52.
Abstract
The nuclear receptor (NR) superfamily, which is divided into 7 subfamilies, constitutes one of the largest classes of transcription factors. In this study, through comprehensive database search, we identified all NRs (including 4 novel members) from the tilapia (75), common carp (137), zebrafish (73), fugu (73), tetraodon (72), stickleback (70), medaka (69), coelacanth (55), spotted gar (51) and elephant shark (50). For 21 NRs, two duplicates were found in teleosts, while only one in tetrapods. These duplicates, except those of DAX1, SHP and GCNF found in the elephant shark, were derived from 3R (third round of genome duplication). The linkage duplication of 5 syntenic blocks (comprising 14 duplicated NR couples) in teleosts further supported their 3R origin. Based on transcriptome data from adult tilapia, 53 NRs were found to be expressed in more than one tissue (brain, head kidney, heart, liver, kidney, muscle, ovary and testis), and 4 were tissue-specific, indicating their essential roles in the corresponding tissue. Based on the XX and XY gonadal transcriptome data from four developmental stages, 65 NRs were detected in gonads, with 21, 31, 11 and 29 expressed sexual dimorphically at 5, 30, 90 and 180 days after hatching, respectively. The expression of four selected genes was examined by in situ hybridization (ISH) and quantitative PCR (qPCR) to validate the spatial and temporal expression profiles of NRs. Comparative analyses of the expression profiles of duplicated NRs revealed divergence in gene expression as well as gene function. Our results demonstrated that NRs may play important roles in sex determination and gonadal development in teleosts.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping