PUBLICATION
A Novel Method for Rearing Zebrafish by Using Freshwater Rotifers (Brachionus calyciflorus)
- Authors
- Aoyama, Y., Moriya, N., Tanaka, S., Taniguchi, T., Hosokawa, H., Maegawa, S.
- ID
- ZDB-PUB-150506-14
- Date
- 2015
- Source
- Zebrafish 12(4): 288-95 (Journal)
- Registered Authors
- Maegawa, Shingo
- Keywords
- none
- MeSH Terms
-
- Animal Feed/analysis
- Animals
- Aquaculture/methods*
- Artemia
- Diet*
- Female
- Longevity
- Male
- Rotifera*
- Time Factors
- Zebrafish/growth & development
- Zebrafish/physiology*
- PubMed
- 25938499 Full text @ Zebrafish
Citation
Aoyama, Y., Moriya, N., Tanaka, S., Taniguchi, T., Hosokawa, H., Maegawa, S. (2015) A Novel Method for Rearing Zebrafish by Using Freshwater Rotifers (Brachionus calyciflorus). Zebrafish. 12(4):288-95.
Abstract
The zebrafish (Danio rerio) has become a powerful model organism for studying developmental processes and genetic diseases. However, there remain several problems in previous rearing methods. In this study, we demonstrate a novel method for rearing zebrafish larvae by using a new first food, freshwater rotifers (Brachionus calyciflorus). Feeding experiments indicated that freshwater rotifers are suitable as the first food for newly hatched larval fish. In addition, we revisited and improved a feeding schedule from 5 to 40 days postfertilization. Our feeding method using freshwater rotifers accelerated larval growth. At 49 dpf, one pair out of 10 pairs successfully produced six fertilized eggs. At 56, 63, and 71 dpf, 6 out of the 10 pairs constantly produced normal embryos. Our method will improve the husbandry of the zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping