ZFIN ID: ZDB-PUB-150412-4
Molecular cloning and expression analysis of an apoptosis-associated gene Daxx from zebrafish, Danio rerio
Qi, L., Xiang, Z.
Date: 2015
Source: Fish & shellfish immunology 45(1): 59-66 (Journal)
Registered Authors:
Keywords: Apoptosis, Daxx, ISKNV, Innate immunity, Zebrafish
MeSH Terms:
  • Adaptor Proteins, Signal Transducing/chemistry
  • Adaptor Proteins, Signal Transducing/genetics*
  • Adaptor Proteins, Signal Transducing/metabolism
  • Amino Acid Sequence
  • Animals
  • Apoptosis*
  • Cloning, Molecular
  • DNA Virus Infections/genetics
  • DNA Virus Infections/veterinary*
  • DNA Virus Infections/virology
  • DNA, Complementary/genetics
  • DNA, Complementary/metabolism
  • Fish Diseases/genetics*
  • Fish Diseases/virology
  • Gene Expression Profiling
  • Gene Expression Regulation*
  • Humans
  • Iridoviridae/physiology*
  • Nuclear Proteins/chemistry
  • Nuclear Proteins/genetics*
  • Nuclear Proteins/metabolism
  • Phylogeny
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Zebrafish*/genetics
  • Zebrafish*/metabolism
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 25862973 Full text @ Fish Shellfish Immunol.
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ABSTRACT
The death domain-associated protein Daxx exerts many functions including the induction and inhibition of apoptosis, regulation of chromatin remodeling and gene transcription. In this report, we have cloned and characterized a Daxx ortholog from the zebrafish, Danio rerio. The bioinformatics analysis results indicated that the open reading frame (ORF) of zebrafish Daxx is 2,151bp long and encodes a putative protein of 716 amino acids containing Daxx domain. Though quantitative PCR analyses, Daxx mRNA was detected in embryonic development from 6 h to120 h and in all 11 selected zebrafish tissues, and the expression of Daxx was increased first and then decreased during megalocytivirus infectious spleen and kidney necrosis virus (ISKNV) infection. Fluorescence microscopy indicated that the full-length protein was located in the nuclei of the tested Hela cells uniformly but punctiform distribution in HEK293T. In the luciferase report assays, the GAL4-Daxx fusion protein inhibited the transcriptional activity of L8G5-Luc reporter gene showed that Daxx might act as a transcriptional repressor, following the over-expression in HEK293T, the activation of NF-κB-Luc and p53/p21-Luc reporter genes were repressed by the protein. These results suggested that Daxx might play definite role in apoptosis and innate immunity in zebrafish.
ADDITIONAL INFORMATIONNo data available