ZFIN ID: ZDB-PUB-150317-4
An In Vivo Requirement for the Mediator Subunit Med14 in the Maintenance of Stem Cell Populations
Burrows, J.T., Pearson, B.J., Scott, I.C.
Date: 2015
Source: Stem Cell Reports   4(4): 670-84 (Journal)
Registered Authors: Burrows, Jeff, Pearson, Bret, Scott, Ian
Keywords: none
Microarrays: GEO:GSE58042
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation/genetics
  • Cell Self Renewal/genetics*
  • Gene Expression
  • Immunohistochemistry
  • Mediator Complex/chemistry
  • Mediator Complex/genetics*
  • Mediator Complex/metabolism*
  • Mutation
  • Phenotype
  • Protein Subunits/genetics*
  • Protein Subunits/metabolism*
  • Stem Cells/cytology*
  • Stem Cells/metabolism*
  • Zebrafish
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism*
PubMed: 25772472 Full text @ Stem Cell Reports
The Mediator complex has recently been shown to be a key player in the maintenance of embryonic and induced pluripotent stem cells. However, the in vivo consequences of loss of many Mediator subunits are unknown. We identified med14 as the gene affected in the zebrafish logelei (log) mutant, which displayed a morphological arrest by 2 days of development. Surprisingly, microarray analysis showed that transcription was not broadly affected in log mutants. Indeed, log cells transplanted into a wild-type environment were able to survive into adulthood. In planarians, RNAi knockdown demonstrated a requirement for med14 and many other Mediator components in adult stem cell maintenance and regeneration. Multiple stem/progenitor cell populations were observed to be reduced or absent in zebrafish med14 mutant embryos. Taken together, our results show a critical, evolutionarily conserved, in vivo function for Med14 (and Mediator) in stem cell maintenance, distinct from a general role in transcription.