|ZFIN ID: ZDB-PUB-150204-8|
miR-216a regulates snx5, a novel notch signaling pathway component, during zebrafish retinal development
Olena, A.F., Rao, M.B., Thatcher, E.J., Wu, S.Y., Patton, J.G.
|Source:||Developmental Biology 400(1): 72-81 (Journal)|
|Registered Authors:||Patton, James G., Thatcher, Elizabeth, Wu, Shu-Yu (Simon)|
|Keywords:||Microrna, Mir-216a, Notch signaling, Retina, Sorting nexin 5, Zebrafish|
|PubMed:||25645681 Full text @ Dev. Biol.|
Olena, A.F., Rao, M.B., Thatcher, E.J., Wu, S.Y., Patton, J.G. (2015) miR-216a regulates snx5, a novel notch signaling pathway component, during zebrafish retinal development. Developmental Biology. 400(1):72-81.
ABSTRACTPrecise regulation of Notch signaling is essential for normal vertebrate development. Mind bomb (Mib) is a ubiquitin ligase that is required for activation of Notch by Notch's ligand, Delta. Sorting Nexin 5 (SNX5) co-localizes with Mib and Delta complexes and has been shown to directly bind to Mib. We show that microRNA-216a (miR-216a) is expressed in the retina during early development and regulates snx5 to precisely regulate Notch signaling. miR-216a and snx5 have complementary expression patterns. Knocking down miR-216a and/or overexpression of snx5 resulted in increased Notch activation. Conversely, knocking down snx5 and/or miR-216a overexpression caused a decrease in Notch activation. We propose a model in which SNX5, precisely controlled by miR-216a, is a vital partner of Mib in promoting endocytosis of Delta and subsequent activation of Notch signaling.