ZFIN ID: ZDB-PUB-150131-1
A DN-mda5 transgenic zebrafish model demonstrates that Mda5 plays an important role in snakehead rhabdovirus resistance
Gabor, K., Charette, J., Pietraszewski, M., Wingfield, D., Shim, J., Millard, P., Kim, C.
Date: 2015
Source: Developmental and comparative immunology   51(2): 298-304 (Journal)
Registered Authors: Kim, Carol H.
Keywords: Danio rerio, Type I IFN, Zebrafish, antiviral, mda5, rhabdovirus
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • DEAD-box RNA Helicases/genetics
  • DEAD-box RNA Helicases/immunology
  • DEAD-box RNA Helicases/metabolism*
  • Immunity, Active/genetics
  • Interferon Type I/metabolism
  • Mutation/genetics
  • Receptors, Pattern Recognition/genetics
  • Receptors, Pattern Recognition/immunology
  • Receptors, Pattern Recognition/metabolism*
  • Rhabdoviridae/immunology*
  • Rhabdoviridae Infections/immunology*
  • Transgenes/genetics
  • Viral Load/genetics
  • Zebrafish/immunology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/immunology
  • Zebrafish Proteins/metabolism*
PubMed: 25634485 Full text @ Dev. Comp. Immunol.
ABSTRACT
Melanoma Differentiation-Associated protein 5 (MDA5) is a member of the retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) family, which are cytosolic pattern recognition receptors that detect viral nucleic acids. Here we show an Mda5-dependent response to rhabdovirus infection in vivo using a dominant-negative mda5 transgenic zebrafish. Dominant-negative mda5 zebrafish embryos displayed an impaired antiviral immune response compared to wild-type counterparts that can be rescued by recombinant full-length Mda5. To our knowledge, we have generated the first dominant-negative mda5 transgenic zebrafish and demonstrated a critical role for Mda5 in the antiviral response to rhabdovirus.
ADDITIONAL INFORMATION