|ZFIN ID: ZDB-PUB-150131-1|
A DN-mda5 transgenic zebrafish model demonstrates that Mda5 plays an important role in snakehead rhabdovirus resistance
Gabor, K., Charette, J., Pietraszewski, M., Wingfield, D., Shim, J., Millard, P., Kim, C.
|Source:||Developmental and comparative immunology 51(2): 298-304 (Journal)|
|Registered Authors:||Kim, Carol H.|
|Keywords:||Danio rerio, Type I IFN, Zebrafish, antiviral, mda5, rhabdovirus|
|PubMed:||25634485 Full text @ Dev. Comp. Immunol.|
Gabor, K., Charette, J., Pietraszewski, M., Wingfield, D., Shim, J., Millard, P., Kim, C. (2015) A DN-mda5 transgenic zebrafish model demonstrates that Mda5 plays an important role in snakehead rhabdovirus resistance. Developmental and comparative immunology. 51(2):298-304.
ABSTRACTMelanoma Differentiation-Associated protein 5 (MDA5) is a member of the retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) family, which are cytosolic pattern recognition receptors that detect viral nucleic acids. Here we show an Mda5-dependent response to rhabdovirus infection in vivo using a dominant-negative mda5 transgenic zebrafish. Dominant-negative mda5 zebrafish embryos displayed an impaired antiviral immune response compared to wild-type counterparts that can be rescued by recombinant full-length Mda5. To our knowledge, we have generated the first dominant-negative mda5 transgenic zebrafish and demonstrated a critical role for Mda5 in the antiviral response to rhabdovirus.