PUBLICATION

Akap12 is essential for the morphogenesis of muscles involved in zebrafish locomotion

Authors
Kim, H., Kim, J., Jeong, J., Han, S., Kim, K.
ID
ZDB-PUB-141224-13
Date
2014
Source
Differentiation; research in biological diversity   88(4-5): 106-16 (Journal)
Registered Authors
Keywords
Development, Locomotion, Morphogenesis, Muscle, akap12
MeSH Terms
  • A Kinase Anchor Proteins/genetics
  • A Kinase Anchor Proteins/metabolism*
  • Animals
  • Morphogenesis*
  • Muscle, Skeletal/embryology
  • Muscle, Skeletal/metabolism*
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Swimming*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
25534553 Full text @ Differentiation
Abstract
Swimming behavior in fish is driven by coordinated contractions of muscle fibers. In zebrafish, slow muscle cell migration is crucial for the formation of the muscle network; slow myoblasts, which arise from medial adaxial cells, migrate radially to the lateral surface of the trunk and tail during embryogenesis. This study found that the zebrafish A-kinase anchoring protein (akap)12 isoforms akap12α and akap12β are required for muscle morphogenesis and locomotor activity. Embryos deficient in akap12 exhibited reduced spontaneous coiling, touch response, and free swimming. Akap12-depleted slow but not fast muscle cells were misaligned, suggesting that the behavioral abnormalities resulted from specific defects in slow muscle patterning; indeed, slow muscle cells and muscle pioneers in these embryos showed abnormal migration in a cell-autonomous manner. Taken together, these results suggest that akap12 plays a critical role in the development of zebrafish locomotion by regulating the normal morphogenesis of muscles.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping