PUBLICATION
Retinal vasculature of adult zebrafish: In vivo imaging using confocal scanning laser ophthalmoscopy
- Authors
- Bell, B.A., Xie, J., Yuan, A., Kaul, C., Hollyfield, J.G., Anand-Apte, B.
- ID
- ZDB-PUB-141203-59
- Date
- 2014
- Source
- Experimental Eye Research 129C: 107-118 (Journal)
- Registered Authors
- Anand-Apte, Bela, Xie, Jing
- Keywords
- Blood retinal barrier, Confocal, Imaging, Retina, SLO, Scanning laser ophthalmoscope, Vasculature, Zebrafish
- MeSH Terms
-
- Animals
- Capillary Permeability*
- Diagnostic Imaging/methods*
- Fundus Oculi
- Ophthalmoscopy/methods*
- Retinal Vessels/cytology*
- Zebrafish
- PubMed
- 25447564 Full text @ Exp. Eye. Res.
Citation
Bell, B.A., Xie, J., Yuan, A., Kaul, C., Hollyfield, J.G., Anand-Apte, B. (2014) Retinal vasculature of adult zebrafish: In vivo imaging using confocal scanning laser ophthalmoscopy. Experimental Eye Research. 129C:107-118.
Abstract
Over the past 3 decades the zebrafish (Danio rerio) has become an important biomedical research species. As their use continues to grow additional techniques and tools will be required to keep pace with ongoing research using this species. In this paper we describe a novel method for in vivo imaging of the retinal vasculature in adult animals using a commercially available confocal scanning laser ophthalmoscope (SLO). With this instrumentation, we demonstrate the ability to distinguish diverse vascular phenotypes in different transgenic GFP lines. In addition this technology allows repeated visualization of the vasculature in individual zebrafish over time to document vascular leakage progression and recovery induced by intraocular delivery of proteins that induce vascular permeability. SLO of the retinal vasculature was found to be highly informative, providing images of high contrast and resolution that were capable of resolving individual vascular endothelial cells. Finally, the procedures required to acquire SLO images from zebrafish are non-invasive, simple to perform and can be achieved with low animal mortality, allowing repeated imaging of individual fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping