PUBLICATION

Pten function in zebrafish: anything but a fish story

Authors
Stumpf, M., Choorapoikayil, S., den Hertog, J.
ID
ZDB-PUB-141203-19
Date
2015
Source
Methods (San Diego, Calif.)   77-78: 191-6 (Review)
Registered Authors
Choorapoikayil, Suma, den Hertog, Jeroen
Keywords
Angiogenesis, Hemangiosarcoma, Hematopoiesis, PTEN, Zebrafish
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Humans
  • Models, Animal*
  • Molecular Sequence Data
  • PTEN Phosphohydrolase/physiology*
  • Species Specificity
  • Tumor Suppressor Proteins/physiology*
  • Zebrafish
  • Zebrafish Proteins/physiology*
PubMed
25461815 Full text @ Methods
Abstract
Zebrafish is an excellent model system for the analysis of gene function. We and others use zebrafish to investigate the function of the tumor suppressor, Pten, in tumorigenesis and embryonic development. Zebrafish have two pten genes, ptena and ptenb. The recently identified N-terminal extension of human PTEN that may facilitate cell membrane transfer, appears not to be conserved in zebrafish Ptena or Ptenb. Mutants that retain a single wild type pten allele develop tumors, predominantly hemangiosarcomas. Homozygous double mutants are embryonic lethal. Zebrafish embryos lacking functional Pten display enhanced proliferation of endothelial cells, resulting in hyperbranching of blood vessels. In addition, ptena-/-ptenb-/- mutant embryos display enhanced proliferation of hematopoietic stem and progenitor cells and concomitant arrest of differentiation, although Pten-deficient cells commit to all blood cell lineages. Zebrafish is an ideal model for intravital imaging and future work using ptena-/-ptenb-/- mutants will enhance our understanding of the function of Pten in vivo.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping