PUBLICATION

Histopathological lesions, P-glycoprotein and PCNA expression in zebrafish (Danio rerio) liver after a single exposure to diethylnitrosamine

Authors
Machado, S.P., Cunha, V., Reis-Henriques, M.A., Ferreira, M.
ID
ZDB-PUB-141010-1
Date
2014
Source
Environmental Toxicology and Pharmacology   38: 720-732 (Journal)
Registered Authors
Keywords
Hepatocarcinogens, Immunohistochemistry, P-gp, PCNA, Zebrafish
MeSH Terms
  • ATP Binding Cassette Transporter, Subfamily B/metabolism*
  • Animals
  • Carcinogens/administration & dosage*
  • Carcinogens/pharmacology
  • Diethylnitrosamine/administration & dosage*
  • Diethylnitrosamine/pharmacology
  • Female
  • Gene Expression Regulation, Neoplastic/drug effects
  • Liver/metabolism
  • Liver/pathology*
  • Male
  • Proliferating Cell Nuclear Antigen/metabolism*
  • Zebrafish
PubMed
25299848 Full text @ Environ. Toxicol. Pharmacol.
Abstract
The presence of carcinogenic compounds in the aquatic environment is a recognized problem. ABC transporters are well known players in the multidrug-resistance (MDR) phenomenon in mammals associated with resistance to chemotherapy, however little is known in fish species. Thus, the aim of this study was to induce hepatic tumours and evaluate long-term effects on P-glycoprotein (P-gp) and proliferating cell nuclear antigen (PCNA) proteins in Danio rerio liver, after exposure to diethylnitrosamine (DEN). Several hepatic histopathological alterations were observed in zebrafish after exposure to DEN including pre-neoplastic lesions 6 and 9 months post-exposure. After 3, 6 and 9 months of exposure to DEN, P-gp and PCNA proteins expression were up-regulated. In conclusion, this study has shown that zebrafish ABC transporters can play a similar role as in human disease, hence zebrafish can be used also as a biological model to investigate in more deep mechanisms involved in disease processes.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping