Retinoic acid negatively regulates dact3b expression in the hindbrain of zebrafish embryos

Mandal, A., Waxman, J.
Gene expression patterns : GEP   16(2): 122-9 (Journal)
Registered Authors
Waxman, Joshua
Dact, Dishevelled, Wnt signaling, retinoic acid signaling, vertebrate development
MeSH Terms
  • Adaptor Proteins, Signal Transducing/genetics
  • Adaptor Proteins, Signal Transducing/metabolism*
  • Animals
  • Embryonic Development/drug effects
  • Embryonic Development/genetics
  • Gene Expression Regulation, Developmental
  • In Situ Hybridization
  • Neural Crest/drug effects
  • Neural Crest/embryology
  • Neural Crest/metabolism
  • Neurons/drug effects
  • Neurons/metabolism
  • Nuclear Proteins/genetics
  • Nuclear Proteins/metabolism*
  • Rhombencephalon/drug effects
  • Rhombencephalon/embryology
  • Rhombencephalon/metabolism
  • Signal Transduction
  • Somites/drug effects
  • Somites/embryology
  • Somites/metabolism
  • Tretinoin/pharmacology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
25266145 Full text @ Gene Expr. Patterns
Wnt signaling plays important roles in normal development as well as pathophysiological conditions. The Dapper antagonist of β-catenin (Dact) proteins are modulators of both canonical and non-canonical Wnt signaling via direct interactions with Dishevelled (Dvl) and Van Gogh like-2 (Vangl2). Here, we report the dynamic expression patterns of two zebrafish dact3 paralogs during early embryonic development. Our whole mount in situ┬áhybridization (WISH) analysis indicates that specific dact3a expression starts by the tailbud stage in adaxial cells. Later, it is expressed in the anterior lateral plate mesoderm, somites, migrating cranial neural crest, and hindbrain neurons. By comparison, dact3b expression initiates on the dorsal side at the dome stage and soon after is expressed in the dorsal forerunner cells (DFCs) during gastrulation. At later stages, dact3b expression becomes restricted to the branchial neurons of the hindbrain and to the 2(nd) pharyngeal arch. To investigate how zebrafish dact3 gene expression is regulated, we manipulated retinoic acid (RA) signaling during development and found it negatively regulates dact3b in the hindbrain. Our study is the first to document the expression of the paralogous zebrafish dact3 genes during early development and demonstrate dact3b can be regulated by RA signaling. Therefore, our study opens up new avenues to study Dact3 function in the development of multiple tissues and suggests a previously unappreciated cross regulation of Wnt signaling by RA signaling in the developing vertebrate hindbrain.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes