PUBLICATION
Chevron formation of the zebrafish muscle segments
- Authors
- Rost, F., Eugster, C., Schröter, C., Oates, A.C., Brusch, L.
- ID
- ZDB-PUB-141001-3
- Date
- 2014
- Source
- The Journal of experimental biology 217(Pt 21): 3870-82 (Journal)
- Registered Authors
- Oates, Andrew
- Keywords
- none
- MeSH Terms
-
- Animals
- In Situ Hybridization
- Models, Biological
- Morphogenesis/physiology*
- Muscle Contraction/physiology
- Muscle Tonus
- Muscle, Skeletal/embryology*
- Somites/embryology*
- Time-Lapse Imaging
- Veratrum Alkaloids
- Zebrafish/embryology*
- PubMed
- 25267843 Full text @ J. Exp. Biol.
Citation
Rost, F., Eugster, C., Schröter, C., Oates, A.C., Brusch, L. (2014) Chevron formation of the zebrafish muscle segments. The Journal of experimental biology. 217(Pt 21):3870-82.
Abstract
The muscle segments of fish have a folded shape, termed a chevron, which is thought to be optimal for the undulating body movements of swimming. However, the mechanism shaping the chevron during embryogenesis is not understood. Here, we use time-lapse microscopy of developing zebrafish embryos spanning the entire somitogenesis period to quantitate the dynamics of chevron shape development. Comparing such time courses with the start of movements in wildtype zebrafish and analyzing immobile mutants, we show that the previously implicated body movements do not play a role in chevron formation. Further, the monotonic increase of chevron angle along the anteroposterior axis revealed by our data constrains or rules out possible contributions by previously proposed mechanisms. In particular, we find that muscle pioneers are not required for chevron formation. We put forward a tension-and-resistance mechanism involving interactions between intra-segmental tension and segment boundaries. To evaluate this mechanism, we derive and analyze a mechanical model of a chain of contractile and resisting elements. The predictions of this model are verified by comparison to experimental data. Altogether, our results support the notion that a simple physical mechanism suffices to self-organize the observed spatiotemporal pattern in chevron formation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping