PUBLICATION

Clonal Analysis of kit ligand a Functional Expression Reveals Lineage-Specific Competence to Promote Melanocyte Rescue in the Mutant Regenerating Caudal Fin

Authors
Tryon, R.C., Johnson, S.L.
ID
ZDB-PUB-140712-12
Date
2014
Source
PLoS One   9: e102317 (Journal)
Registered Authors
Johnson, Stephen L.
Keywords
none
MeSH Terms
  • Animal Fins/growth & development*
  • Animals
  • Cell Differentiation/genetics*
  • Cell Lineage/genetics
  • Fibroblasts/cytology
  • Gene Expression Regulation, Developmental
  • Melanocytes/metabolism
  • Osteoblasts/cytology
  • Promoter Regions, Genetic
  • Regeneration/genetics*
  • Stem Cell Factor/biosynthesis*
  • Stem Cell Factor/genetics
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics
PubMed
25009992 Full text @ PLoS One
Abstract
The study of regeneration in an in vivo vertebrate system has the potential to reveal targetable genes and pathways that could improve our ability to heal and repair damaged tissue. We have developed a system for clonal labeling of discrete cell lineages and independently inducing gene expression under control of the heat shock promoter in the zebrafish caudal fin. Consequently we are able to test the affects of overexpressing a single gene in the context of regeneration within each of the nine different cell lineage classes that comprise the caudal fin. This can test which lineage is necessary or sufficient to provide gene function. As a first example to demonstrate this approach, we explored which lineages were competent to functionally express the kit ligand a protein as assessed by the local complementation of the mutation in the sparse-like (kitlgatc244b) background. We show that dermal fibroblast expression of kit ligand a robustly supports the rescue of melanocytes in the regenerating caudal fin. kit ligand a expression from skin and osteoblasts results in more modest and variable rescue of melanocytes, while lateral line expression was unable to complement the mutation.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping