PUBLICATION
In Vivo Optogenetics for Light-Induced Oxidative Stress in Transgenic Zebrafish Expressing the KillerRed Photosensitizer Protein
- Authors
- Teh, C., Korzh, V.
- ID
- ZDB-PUB-140513-171
- Date
- 2014
- Source
- Methods in molecular biology (Clifton, N.J.) 1148: 229-38 (Chapter)
- Registered Authors
- Korzh, Vladimir, Teh, Cathleen
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Female
- Gene Expression
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Heart Rate
- Larva/genetics
- Larva/metabolism
- Larva/radiation effects
- Light
- Male
- Myocardial Contraction
- Myocardium/metabolism
- Myocardium/pathology
- Optogenetics*
- Oxidative Stress/radiation effects*
- Photosensitizing Agents/metabolism
- Ventricular Function
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 24718805 Full text @ Meth. Mol. Biol.
Citation
Teh, C., Korzh, V. (2014) In Vivo Optogenetics for Light-Induced Oxidative Stress in Transgenic Zebrafish Expressing the KillerRed Photosensitizer Protein. Methods in molecular biology (Clifton, N.J.). 1148:229-38.
Abstract
Optogenetic methods are gaining broad recognition. The zebrafish is particularly useful for these applications as a model vertebrate due to a unique combination of translucent embryos/larvae and efficient transgenesis. Here, we describe a zebrafish model of light-induced cardiac deficiency. Upon illumination with intense green light, the membrane-tethered photosensitizer protein KillerRed acts as a photoinducer of reactive oxygen species which in turn cause changes in heart rate and contractility in hearts that express this transgene.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping