PUBLICATION

Analyzing craniofacial morphogenesis in zebrafish using 4D confocal microscopy

Authors
McGurk, P.D., Lovely, C.B., and Eberhart, J.K.
ID
ZDB-PUB-140429-9
Date
2014
Source
Journal of visualized experiments : JoVE   (83): e51190 (Journal)
Registered Authors
Eberhart, Johann
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Craniofacial Abnormalities/genetics
  • Facial Bones/embryology*
  • Microscopy, Confocal/methods*
  • Morphogenesis/genetics
  • Morphogenesis/physiology
  • Neural Crest/embryology
  • Receptor, Platelet-Derived Growth Factor alpha/genetics
  • Receptors, G-Protein-Coupled/genetics
  • Smad5 Protein/genetics
  • Time-Lapse Imaging/methods
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
PubMed
24514435 Full text @ J. Vis. Exp.
Abstract

Time-lapse confocal imaging is a powerful technique useful for characterizing embryonic development. Here, we describe the methodology and characterize craniofacial morphogenesis in wild-type, as well as pdgfra, smad5, and smo mutant embryos.

Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping