In vitro analysis suggests that difference in cell movement during direct interaction can generate various pigment patterns in vivo
- Authors
- Yamanaka, H., and Kondo, S.
- ID
- ZDB-PUB-140321-17
- Date
- 2014
- Source
- Proceedings of the National Academy of Sciences of the United States of America 111(5): 1867-1872 (Journal)
- Registered Authors
- Kondo, Shigeru
- Keywords
- none
- MeSH Terms
-
- Animal Fins/cytology
- Animals
- Cell Communication*
- Cell Movement*
- Cell Separation
- Melanophores/cytology
- Melanophores/metabolism
- Mice
- Models, Biological
- Mutation/genetics
- Pigmentation*
- Statistics as Topic
- Zebrafish/physiology*
- PubMed
- 24449859 Full text @ Proc. Natl. Acad. Sci. USA
Pigment patterns of organisms have invoked strong interest from not only biologists but also, scientists in many other fields. Zebrafish is a useful model animal for studying the mechanism of pigment pattern formation. The zebrafish stripe pattern is primarily two types of pigment cells: melanophores and xanthophores. Previous studies have reported that interactions among these pigment cells are important for pattern formation. In the recent report, we found that the direct contact by xanthophores induces the membrane depolarization of melanophores. From analysis of jaguar mutants, it is suggested that the depolarization affects the movements of melanophores. To analyze the cell movement in detail, we established a unique in vitro system. It allowed us to find that WT xanthophores induced repulsive movement of melanophores through direct contact. The xanthophores also chased the melanophores. As a result, they showed run-and-chase movements. We also analyzed the cell movement of pigment cells from jaguar and leopard mutants, which have fuzzy stripes and spot patterns, respectively. jaguar cells showed inhibited run-and-chase movements, and leopard melanophores scarcely showed repulsive response. Furthermore, we paired mutant and WT cells and showed which of the melanophores and xanthophores have responsibility for the altered cell movements. These results suggested that there is a correspondence relationship between the cell movements and pigment patterns. The correspondence relationship highlighted the importance of the cell movements in the pattern formation and showed that our system is a quite useful system for future study in this field.