Analysis of three mu-AP1 subunits during zebrafish development
- Authors
- Gariano, G., Guarienti, M., Bresciani, R., Borsani, G., Carola, G., Monti, E., Giuliani, R., Rezzani, R., Bonomini, F., Preti, A., Schu, P., and Zizioli, D.
- ID
- ZDB-PUB-131115-9
- Date
- 2014
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 243(2): 299-314 (Journal)
- Registered Authors
- Borsani, Giuseppe
- Keywords
- adaptin, central nervous system, development, kidney, vesicular transport, zebrafish
- MeSH Terms
-
- Acridine Orange
- Adaptor Protein Complex 1/genetics
- Adaptor Protein Complex 1/metabolism
- Adaptor Protein Complex beta Subunits/genetics
- Adaptor Protein Complex beta Subunits/metabolism
- Adaptor Protein Complex mu Subunits/genetics
- Adaptor Protein Complex mu Subunits/metabolism*
- Animals
- Base Sequence
- DNA Primers/genetics
- Embryonic Development/genetics*
- Gene Knockdown Techniques
- In Situ Hybridization
- Molecular Sequence Data
- Morpholinos/genetics
- Phylogeny
- Protein Subunits/genetics
- Protein Transport/genetics
- Protein Transport/physiology
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Alignment
- Sequence Analysis
- Zebrafish/embryology*
- Zebrafish/metabolism
- trans-Golgi Network/metabolism*
- PubMed
- 24123392 Full text @ Dev. Dyn.
Background: The family of AP-1 complexes mediates protein sorting in the late secretory pathway and it is essential for the development of mammals. The ubiquitously expressed AP-1A complex consists of four adaptins γ1, β1, μ1A and σ1A. AP-1A mediates protein transport between the trans-Golgi network and early endosomes The polarized epithelia AP-1B complex contains the μ1B-adaptin. AP-1B mediates specific transport of proteins from basolateral recycling endosomes to the basolateral plasma membrane of polarized epithelial cells. Results: Analysis of the zebrafish genome revealed the existence of three 1-adaptin genes, encoding 1A, 1B and the novel isoform μ1C, which is not found in mammals. μ1C shows 80% sequence identity with 1A and 1B. The 1C expression pattern largely overlaps with that of 1A, while 1B is expressed in epithelial cells. By knocking-down the synthesis of 1A, 1B and 1C with antisense morpholino techniques we demonstrate that each of these 1 adaptins is essential for zebrafish development, with 1A and 1C being involved in central nervous system development and 1B in kidney formation. Conclusions: Zebrafish is unique in expressing three AP-1 complexes: AP-1A, AP-1B and AP-1C. Our results demonstrate that they are not redundant and that each of them has specific functions, which can not be fulfilled by one of the other isoforms. Each of the 1 adaptins appears to mediate specific molecular mechanisms essential for early developmental processes, which depend on specific intracellular vesicular protein sorting pathways.