PUBLICATION
A molecular method for assessing the effects of potential contaminants on the rate of zebrafish (Danio rerio) development
- Authors
- Beasley, A., Graham, C., Otter, R., and Elrod-Erickson, M.
- ID
- ZDB-PUB-131115-6
- Date
- 2014
- Source
- Environmental toxicology and chemistry 33(1): 238-42 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Embryonic Development/drug effects
- Gene Expression Regulation, Developmental/drug effects*
- Hedgehog Proteins/genetics
- Hot Temperature
- Metal Nanoparticles/toxicity*
- SOX Transcription Factors/genetics
- Silver/toxicity*
- Stress, Physiological
- Troponin T/genetics
- Zebrafish/genetics*
- Zebrafish/growth & development
- Zebrafish Proteins/genetics
- PubMed
- 24122956 Full text @ Environ. Toxicol. Chem.
Citation
Beasley, A., Graham, C., Otter, R., and Elrod-Erickson, M. (2014) A molecular method for assessing the effects of potential contaminants on the rate of zebrafish (Danio rerio) development. Environmental toxicology and chemistry. 33(1):238-42.
Abstract
Monitoring expression of the developmentally regulated genes shh, sox2, and tnnt1 by reverse transcriptase polymerase chain reaction (RT-PCR) allows determination of the rate of embryogenesis in zebrafish (Danio rerio) embryos without direct visual observation. The utility of combining this approach and morphological methods during toxicity studies was demonstrated with embryos developing at either 28.5 °C or 24.5 °C, and with embryos exposed to sublethal doses of silver nanoparticles.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping