PUBLICATION

Nonmuscle myosin II-B (myh10) expression analysis during zebrafish embryonic development

Authors
Huang, Y., Wang, X., Wang, X., Xu, M., Liu, M., and Liu, D.
ID
ZDB-PUB-130610-56
Date
2013
Source
Gene expression patterns : GEP   13(7): 265-270 (Journal)
Registered Authors
Keywords
NM II, zebrafish, neural system, myh10, myh9a, myh9b, expression
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Central Nervous System/cytology
  • Central Nervous System/embryology
  • Central Nervous System/metabolism*
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development/genetics
  • Epidermis/cytology
  • Epidermis/embryology
  • Epidermis/metabolism*
  • Gene Expression Regulation, Developmental
  • Heart/embryology
  • Heart/physiology
  • Humans
  • In Situ Hybridization, Fluorescence
  • Mice
  • Molecular Sequence Data
  • Myosin Heavy Chains/chemistry
  • Myosin Heavy Chains/genetics*
  • Myosin Heavy Chains/metabolism
  • Nonmuscle Myosin Type IIB/chemistry
  • Nonmuscle Myosin Type IIB/genetics*
  • Nonmuscle Myosin Type IIB/metabolism
  • Olfactory Bulb/cytology
  • Olfactory Bulb/enzymology
  • Olfactory Bulb/metabolism
  • Phylogeny
  • Rats
  • Sequence Alignment
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed
23665442 Full text @ Gene Expr. Patterns
Abstract

Nonmuscle myosin II (NM II) is the name given to the multi-subunit protein product of three genes (myh9, myh10, and myh14) encoding different nonmuscle myosin heavy chains. The three NM II isoforms share a very similar molecular structure and play important roles in a variety of fundamental biological processes. NM II-B (myh10) has been shown to be essential for the formation of mouse neural system and heart. But so far the complete knowledge for its expression in developing zebrafish embryos is lacking. In current study, we proved the conservation of zebrafish NM II-B in vertebrate evolution by in silicon analysis. Afterwards the NM II-B (myh10) expression was demonstrated to initiate after gastrulation stage. At 20 hpf, the expression is mainly restricted in central nervous system (CNS). It was maintained and expanded to sensor organ including eye, otic vesicle, and olfactory bulb at 36 hpf and later. We also detected myh10 mRNA hybridization signal in 48 hpf zebrafish heart. In addition, we investigated myh9a and myh9b mRNA distribution in zebrafish developing embryos. It was shown that myh10 and myh9 have distinct expression pattern, with myh9s not in neural system but in epidermis, enveloping layer (EVL). Our study provides new insight into the NM II expression and the use of this model organism to tackle future studies on the role of NM II in embryo development.

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