PUBLICATION
A Blue-Absorbing Photolabile Protecting Group for in Vivo Chromatically Orthogonal Photoactivation
- Authors
- Fournier, L., Gauron, C., Xu, L., Aujard, I., Le Saux, T., Gagey-Eilstein, N., Maurin, S., Dubruille, S., Baudin, J.B., Bensimon, D., Volovitch, M., Vriz, S., and Jullien, L.
- ID
- ZDB-PUB-130610-1
- Date
- 2013
- Source
- ACS Chemical Biology 8(7): 1528-36 (Journal)
- Registered Authors
- Bensimon, David, Gauron, Carole, Vriz, Sophie
- Keywords
- none
- MeSH Terms
-
- Animals
- Benzhydryl Compounds/chemistry*
- Carbamates/chemistry*
- Color
- Light*
- Microscopy, Confocal
- Molecular Structure
- Staining and Labeling
- Zebrafish/embryology
- PubMed
- 23651265 Full text @ ACS Chem. Biol.
Citation
Fournier, L., Gauron, C., Xu, L., Aujard, I., Le Saux, T., Gagey-Eilstein, N., Maurin, S., Dubruille, S., Baudin, J.B., Bensimon, D., Volovitch, M., Vriz, S., and Jullien, L. (2013) A Blue-Absorbing Photolabile Protecting Group for in Vivo Chromatically Orthogonal Photoactivation. ACS Chemical Biology. 8(7):1528-36.
Abstract
The small and synthetically easily accessible 7-diethylamino-4-thiocoumarinylmethyl photolabile protecting group has been validated for uncaging with blue light. It exhibits a significant action cross-section for uncaging in the 470–500 nm wavelength range and a low light absorption between 350 and 400 nm. These attractive features have been implemented in living zebrafish embryos to perform chromatic orthogonal photoactivation of two biologically active species controlling biological development with UV and blue-cyan light sources, respectively.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping