ZFIN ID: ZDB-PUB-130610-1
A Blue-Absorbing Photolabile Protecting Group for in Vivo Chromatically Orthogonal Photoactivation
Fournier, L., Gauron, C., Xu, L., Aujard, I., Le Saux, T., Gagey-Eilstein, N., Maurin, S., Dubruille, S., Baudin, J.B., Bensimon, D., Volovitch, M., Vriz, S., and Jullien, L.
Date: 2013
Source: ACS Chemical Biology   8(7): 1528-36 (Journal)
Registered Authors: Bensimon, David, Gauron, Carole, Vriz, Sophie
Keywords: none
MeSH Terms:
  • Animals
  • Benzhydryl Compounds/chemistry*
  • Carbamates/chemistry*
  • Color
  • Light*
  • Microscopy, Confocal
  • Molecular Structure
  • Staining and Labeling
  • Zebrafish/embryology
PubMed: 23651265 Full text @ ACS Chem. Biol.

The small and synthetically easily accessible 7-diethylamino-4-thiocoumarinylmethyl photolabile protecting group has been validated for uncaging with blue light. It exhibits a significant action cross-section for uncaging in the 470–500 nm wavelength range and a low light absorption between 350 and 400 nm. These attractive features have been implemented in living zebrafish embryos to perform chromatic orthogonal photoactivation of two biologically active species controlling biological development with UV and blue-cyan light sources, respectively.