PUBLICATION

Perturbation of Invadolysin Disrupts Cell Migration in Zebrafish (Danio rerio)

Authors
Vass, S., and Heck, M.M.
ID
ZDB-PUB-130309-2
Date
2013
Source
Experimental cell research   319(8): 1198-1212 (Journal)
Registered Authors
Keywords
invadolysin, metalloprotease, cell migration, vascular development, zebrafish
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Cell Movement/genetics*
  • Cloning, Molecular
  • Conserved Sequence
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Metalloendopeptidases/genetics
  • Metalloendopeptidases/metabolism
  • Metalloendopeptidases/physiology*
  • Molecular Sequence Data
  • Neovascularization, Physiologic/genetics
  • Tissue Distribution
  • Zebrafish*/embryology
  • Zebrafish*/genetics
  • Zebrafish*/metabolism
  • Zebrafish*/physiology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
PubMed
23422038 Full text @ Exp. Cell Res.
Abstract

Invadolysin is an essential, conserved metalloprotease which links cell division with cell migration and is intriguingly associated with lipid droplets. In this work we examine the expression pattern, protein localisation and gross anatomical consequences of depleting invadolysin in the teleost Danio rerio. We observe that invadolysin plays a significant role in cell migration during development. When invadolysin is depleted by targeted morpholino injection, the appropriate deposition of neuromast clusters and distribution of melanophores are both disrupted. We also observe that blood vessels generated via angiogenesis are affected in invadolysin morphant fish while those formed by vasculogenesis appear normal, demonstrating an unanticipated role for invadolysin in vessel formation. Our results thus highlight a common feature shared by, and a requirement for invadolysin in, these distinct morphological events dependent on cell migration.

Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping