PUBLICATION
Molecular cloning, expression, purification and crystallographic analysis of zebrafish THEM2
- Authors
- Li, H., Gao, F., Yu, S., Jia, M., and Gong, W.
- ID
- ZDB-PUB-121206-12
- Date
- 2012
- Source
- Acta crystallographica. Section F, Structural biology and crystallization communications 68(12): 1525-1528 (Journal)
- Registered Authors
- Keywords
- THEM2, thioesterase superfamily member 2, Danio rerio
- MeSH Terms
-
- Acyl Coenzyme A/chemistry
- Animals
- Cloning, Molecular
- Crystallization
- Crystallography, X-Ray
- Escherichia coli/genetics
- Escherichia coli/metabolism
- Palmitoyl-CoA Hydrolase/chemistry*
- Palmitoyl-CoA Hydrolase/genetics
- Palmitoyl-CoA Hydrolase/isolation & purification
- X-Ray Diffraction
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/chemistry*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/isolation & purification*
- PubMed
- 23192039 Full text @ Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.
Citation
Li, H., Gao, F., Yu, S., Jia, M., and Gong, W. (2012) Molecular cloning, expression, purification and crystallographic analysis of zebrafish THEM2. Acta crystallographica. Section F, Structural biology and crystallization communications. 68(12):1525-1528.
Abstract
Thioesterase superfamily member 2 (THEM2) is essential for cell proliferation of mammalian cells. It belongs to the hotdog-fold thioesterase superfamily and catalyzes the hydrolysis of the thioester bonds of acyl-CoA in vitro. In this study, THEM2 protein from zebrafish (fTHEM2) was expressed in Escherichia coli and purified by Ni-affinity and gel-filtration chromatography. fTHEM2 crystals were obtained using the sitting-drop vapour-diffusion method with PEG 10 000 as precipitant. X-ray diffraction data were collected to 1.80 Å resolution using a synchrotron-radiation source. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 77.1, b = 74.4, c = 96.6 Å, β = 93.7°.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping