PUBLICATION

Action of Vitamin D and the Receptor, VDRa, in Calcium Handling in Zebrafish (Danio rerio)

Authors
Lin, C.H., Su, C.H., Tseng, D.Y., Ding, F.C., and Hwang, P.P.
ID
ZDB-PUB-121012-17
Date
2012
Source
PLoS One   7(9): e45650 (Journal)
Registered Authors
Hwang, Pung Pung, Tseng, Deng-Yu
Keywords
Zebrafish, Embryos, Vitamin D, Gene expression, Vitamins, Vertebrates, Fresh water, Mammals
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Calcium/metabolism*
  • DNA Primers
  • Gene Knockdown Techniques
  • Immunohistochemistry
  • Molecular Sequence Data
  • RNA, Messenger/genetics
  • Real-Time Polymerase Chain Reaction
  • Receptors, Calcitriol/chemistry
  • Receptors, Calcitriol/genetics
  • Receptors, Calcitriol/physiology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Sodium-Potassium-Exchanging ATPase/metabolism
  • TRPV Cation Channels/genetics
  • TRPV Cation Channels/physiology
  • Vitamin D/genetics
  • Vitamin D/physiology*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology
PubMed
23029160 Full text @ PLoS One
Abstract

The purpose of the present study was to use zebrafish as a model to investigate how vitamin D and its receptors interact to control Ca2+ uptake function. Low-Ca2+ fresh water stimulated Ca2+ influx and expressions of epithelial calcium channel (ecac), vitamin D-25-hydroxylase (cyp2r1), vitamin D receptor a (vdra), and vdrb in zebrafish. Exogenous vitamin D increased Ca2+ influx and expressions of ecac and 25-hydroxyvitamin D3-24-hydroxylase (cyp24a1), but downregulated 1α-OHase (cyp27b1) with no effects on other Ca2+ transporters. Morpholino oligonucleotide knockdown of VDRa, but not VDRb, was found as a consequence of calcium uptake inhibition by knockdown of ecac, and ossification of vertebrae is impaired. Taken together, vitamin D-VDRa signaling may stimulate Ca2+ uptake by upregulating ECaC in zebrafish, thereby clarifying the Ca2+-handling function of only a VDR in teleosts. Zebrafish may be useful as a model to explore the function of vitamin D-VDR signaling in Ca2+ homeostasis and the related physiological processes in vertebrates.

Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes